| 不同浓度Cd~(2+)对鲤鱼基因组DNA的影响 |
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| 引用本文： | 曹哲明,杨健.不同浓度Cd~(2+)对鲤鱼基因组DNA的影响[J].应用与环境生物学报,2010(4). |
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| 作者姓名： | 曹哲明 杨健 |
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| 作者单位： | 南京农业大学无锡渔业学院;中国水产科学研究院淡水渔业研究中心内陆渔业环境与资源重点开放实验室; |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金(No.2009JBFB02); 人事部高层次留学人才回国工作项目(No.2-115084)资助~~ |
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| 摘 要： | 鲤鱼(建鲤品种)幼鱼暴露于4个浓度组(0.005、0.05、0.5、5mg/L)的镉离子(Cd~(2+))2d,期间各组的个体均无死亡.用10个RAPD引物对处理后鲤鱼基因组进行扩增,亦未发现有明显的差异扩增条带.但在用甲基化敏感扩增多态性方法研究暴露后鲤鱼肌肉组织的基因组DNA甲基化的变化时发现,5mg/L的Cd~(2+)在d2会使鲤鱼基因组DNA中CCGG区域甲基化发生明显的变化.该组个体1mol后出现大量死亡.另外,0.05mg/L和0.5mg/L Cd~(2+)组个体基因组DNA中CCGG区域甲基化比例2d内略有下降,甲基化位点没有太大改变.说明鲤鱼幼鱼基因组CCGG区域的胞嘧啶甲基化比例在不同浓度Cd~(2+)处理中都比较稳定.低浓度Cd~(2+)条件下鲤鱼主要通过甲基化区域的微调减少Cd~(2+)的生理毒性.高浓度Cd~(2+)处理下,利于基因组DNA甲基化区域发生相当的变化,可能导致一些沉默基因的异常表达.图2表2参31
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| 关 键 词： | 镉 鲤鱼 DNA甲基化 重金属污染 |
Effect of Cadmium at Different Concentrations on Genomic DNA of Common Carps |
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| CAO Zheming, & YANG Jian.Effect of Cadmium at Different Concentrations on Genomic DNA of Common Carps[J].Chinese Journal of Applied and Environmental Biology,2010(4). |
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| Authors: | CAO Zheming & YANG Jian |
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| Institution: | CAO Zheming1,2 & YANG Jian1,2**(1Wuxi Fisheries College,Nanjing Agriculture University,Wuxi 214081,Jiangsu,China)(2Key Laboratory of Ecological Environment and Resources of Inland Fisheries,Freshwater Fisheries Research Centre,Chinese Academy of Fishery Sciences,China) |
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| Abstract: | The 3-month-old Jian common carps(Cyprinus carpio var.Jian) were exposed to various concentrations of Cd~(2+)(0.005,0.05,0.5,5 mg/L) for two days,and no individuals died within the two-day exposure.Also,no significantly amplified loci were found in both treatment and control fishes when ten RAPD primers were detected for the difference in genome DNA.However,when methylation sensitive amplified polymorphism(MSAP) was applied to analyze methylation changes in genome DNA of muscle,significant changes of methyl... |
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| Keywords: | cadmium Jian common carp(Cyprinus carpio var Jian) DNA methylation heavy metal pollution |
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