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| 酵母基因报道系统检测环境二恶英类似物的污染 | |
| 引用本文： | 黄莉,王建华,戴丽军,刘寒英,邱剑峰,文端成,冯媛瑜,李冰.酵母基因报道系统检测环境二恶英类似物的污染[J].应用与环境生物学报,2004,10(4):493-496. |
| 作者姓名： | 黄莉 王建华 戴丽军 刘寒英 邱剑峰 文端成 冯媛瑜 李冰 |
| 作者单位： | 1. 广州医学院实验动物研究中心,广州,510182;西北农林科技大学畜牧兽医学院,陕西,杨凌,712100 2. 西北农林科技大学畜牧兽医学院,陕西,杨凌,712100 3. 广州医学院实验动物研究中心,广州,510182 4. 广州医学院实验医学研究中心,广州,510182 |
| 基金项目： | 浙江省自然科学基金资助项目 (No .30 2 0 56)~~ |
| 摘 要： | 利用一套酵母基因报道检测系统，建立和标化了对二恶英类标准样品测定的方法，证明此系统可检测ng级的二恶英及其类似物，具有简单、快速的的特点．对广州市区工业和生活污水排放较为集中的水体进行的测定显示，二恶英及其类似物在部分地区超标，而各区经处理的自来水均无超标污染．对小鼠急性毒性实验的血清样品的测定同样证明系统能够测定未浓缩处理的复杂样本，具有一定的应用价值．图4参10 |
| 关 键 词： | 二恶英 污染 酵母基因报道系统 检测 |
| 修稿时间： | 2003年11月5日 |
UTILIZATION OF YEAST GENE REPORT SYSTEM TO DETECT ENVIRONMENTAL DIOXINS-LIKE SUBSTANCES |
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| Abstract: | By using a recombinant yeast gene report system, a simple and quick assay of detecting the existence of dioxins was established. It was proved that this protocol could detect dioxins contaminations at ng level. Using this system, the industrial and domestic sewerage waters, as well as tap water in Guangzhou were sampled and detected. The results showed that the sewerage waters were polluted with dioxins in different extents, while the tap waters remained clean. The detection of dioxins in mouse serum samples from acute toxic experiment proved that this system could also be used in complicated samples assay and it has certain advantage over traditional chemical analysis protocols. Fig 4, Ref 10 |
| Keywords: | dioxins contamination yeast gene report system detect |
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