| 植物根瘤内痕量Frankia DNA的提取及鉴定 |
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| 引用本文： | 吴少慧,刘忠,张成刚,张忠泽.植物根瘤内痕量Frankia DNA的提取及鉴定[J].应用与环境生物学报,2001,7(1):76-78. |
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| 作者姓名： | 吴少慧 刘忠 张成刚 张忠泽 |
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| 作者单位： | 中国 科学院沈阳应用生态研究所 |
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| 基金项目： | 国家自然科学基金(No:39870038)资助项目 |
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| 摘 要： | 建立了一种简捷、快速植物根瘤内痕量Frankia DNA的提取方法，即采集自然态下根瘤，剥离瘤瓣，取瘤瓣尖经液氮研主民粉末，以20g/L CTAB（十六烷基三甲基溴化铵）消化胞壁，然后以d=5-10μm的石英粉吸附DNA，再以无菌去离子蒸馏水释放DNA，所获DNA产率及纯度较酚／氯仿法好。经对辽宁沙棘、色赤杨根瘤的瘤内痕量Frankia DNA及体外培养的9种Frankia菌株DNA分纯，并用于酶切及PCR，获满意结果。图3参12
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| 关 键 词： | 根瘤 FRANKIADNA 石英粉 提取 鉴定 DNA提取 |
| 修稿时间： | 2000年6月26日 |
PURIFICATION AND
IDENTIFICATION OF TRACE FRANKIA DNA FROM NODULES |
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| Wu Shaohui,LIU Zhong,Zhang Chenggang,ZHANG Zhongze.PURIFICATION AND
IDENTIFICATION OF TRACE FRANKIA DNA FROM NODULES[J].Chinese Journal of Applied and Environmental Biology,2001,7(1):76-78. |
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| Authors: | Wu Shaohui LIU Zhong Zhang Chenggang ZHANG Zhongze |
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| Abstract: | Actinomycete Frankia can form nitrogen-fixing symbio sis with a large number of woody dicotyledonous plants from different habits and demonstrates great diversity. A simple and rapid protocol was established and d eveloped for purificating trace Frankia DN A from nodules. Frankia in nodules were digested with 20 g/L CTAB and releas ed nucleic acid that were bound to silica particles (d=5～10 μm). Fra nkia DNA from 2 nodules and 9 cultured straing were purified with higher yi elds than those by phenol/chloroform, and could be used for restriction en donucleases and PCR. Fig 3, Ref 12 |
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| Keywords: | nodule Frankia DNA silica purification identification |
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