| 萘降解质粒pND6的分离和鉴定 |
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| 引用本文： | 张心平,岳晓含,黄今勇,牛淑敏,蔡宝立.萘降解质粒pND6的分离和鉴定[J].应用与环境生物学报,2000,6(2):187-190. |
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| 作者姓名： | 张心平 岳晓含 黄今勇 牛淑敏 蔡宝立 |
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| 作者单位： | 南开大学生命科学学院,天津,300071 |
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| 基金项目： | 国家自然科学基金!(No.39670021)，天津市自然科学基金资助项目 |
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| 摘 要： | 从工业废水中分离的假单胞菌 (Pseudomonassp .)ND6菌株 ,能以萘为唯一碳源生长 ,使无机盐培养基(MM)中 2g/L的萘在 48h内降解 98% .该菌株含有一个 115kb的大质粒pND6 .DNA杂交实验表明 ,pND6质粒含有与恶臭假单胞菌 (P .putida)G7菌株的NAH7质粒同源的萘降解基因 .图 3表 1参 14
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| 关 键 词： | 假单胞菌ND6 萘生物降解 质粒 DNA杂交 |
ISOLATION AND IDENTIFICATION OF NAPHTHALENE-DEGRADING PLASMID pND6 |
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| ZHANG Xinping,YUE Xiaohan,HUANG Jinyong,NIU Shumin,CAI Baoli.ISOLATION AND IDENTIFICATION OF NAPHTHALENE-DEGRADING PLASMID pND6[J].Chinese Journal of Applied and Environmental Biology,2000,6(2):187-190. |
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| Authors: | ZHANG Xinping YUE Xiaohan HUANG Jinyong NIU Shumin CAI Baoli |
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| Abstract: | Pseudomonas sp. strain ND6 that was isolated from industrial wastewater is able to grow using naphthalene as sole carbon source and degrade naphthalene of 2 g/L in minimal medium by 98% in 48 hours. This strain contains a large plasmid of 115 kb, pND6. DNA hybridization analysis indicates that pND6 contains naphthalene-degrading genes that are homologous to the genes of naphthalene-degrading plasmid NAH7 from Pseudomonas putida G7. Fig 3, Tab 1, Ref 14 |
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| Keywords: | Pseudomonas sp strain ND6 biodegradation of naphthalene plasmid DNA hybridization |
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