| Xα21基因导入水稻及转基因植株的鉴定 |
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| 引用本文： | 王芋华,毛双林,李浩杰,王世全,李平,罗科.Xα21基因导入水稻及转基因植株的鉴定[J].应用与环境生物学报,2001,7(3):228-231. |
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| 作者姓名： | 王芋华 毛双林 李浩杰 王世全 李平 罗科 |
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| 作者单位： | 1. 中国科学院成都生物研究所
2. 四川农业大学水稻研究所 |
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| 摘 要： | 以水稻愈伤组织为受体、质粒pCXK1301为Xα21基因供体，用农杆菌EHA105和PDS－1000／He基因枪转化受体，经Hyg浓度梯度筛选，获得一些转基因水稻植株，GUS检测、PCR扩增和Southern杂交分析表明，报告基因（GUS）、选择标记基因（Hyg^r）和抗白叶枯病基因Xα21都已整合入转化水稻基因组中，Southern杂分析显示了Xα21基因在转化水稻基因组中整合的拷贝数。田间接菌鉴定表现抗白叶枯病。
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| 关 键 词： | Xα21基因 农杆菌 基因枪 转化 水稻 转基因植株 |
| 修稿时间： | 2000年12月21 |
INTRODUCTION OF BACTERIAL BLIGHT RESISTANCE GENE Xa21 AND MOLECULAR DETECTION IN
TRANSGENIC RICE |
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| WANG Yuhua ,MAO Shuanglin ,LI Haojie ,WANG Shiquan ,LUO Ke ,LI Ping.INTRODUCTION OF BACTERIAL BLIGHT RESISTANCE GENE Xa21 AND MOLECULAR DETECTION IN
TRANSGENIC RICE[J].Chinese Journal of Applied and Environmental Biology,2001,7(3):228-231. |
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| Authors: | WANG Yuhua MAO Shuanglin LI Haojie WANG Shiquan LUO Ke LI Ping |
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| Institution: | WANG Yuhua 1,2,MAO Shuanglin 2,LI Haojie 2,WANG Shiquan 2,LUO Ke 1,LI Ping 2* |
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| Abstract: | pCXK1301 with Xa21 gene used as donar DNA,and the calli induced from mature embryos, young embryos and young panicles as recipient were transformed with microprojectile and Agrobacterium tumefaciens strain EHA105 ,respectively.After gradient selection with Hyg(hygromycin) and antibiotic,GUS detection and PCR analysis,the foreign genes were transformed into the calli,and the regeneration plants were obtained. Southern analysis verified the copies intergrated into rice chromosome.Some transgenic plants were found with strong bacterial blight resistance in the field. Fig 4, Ref 13 |
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| Keywords: | Xa21 gene Agrobacterium tumefaciens microprojectile transformation |
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