首页 | 本学科首页   官方微博 | 高级检索  
     检索      

Zn2+胁迫及恢复下番茄幼苗根系中抗氧化系统的动态变化
引用本文:丁海东,朱为民,杨少军,杨晓峰.Zn2+胁迫及恢复下番茄幼苗根系中抗氧化系统的动态变化[J].应用与环境生物学报,2005,11(5):531-535.
作者姓名:丁海东  朱为民  杨少军  杨晓峰
作者单位:1. 南京农业大学生命科学院,南京,210095;上海市设施园艺技术重点实验室
2. 上海市设施园艺技术重点实验室;上海市农业科学院园艺研究所,上海,201106
3. 上海市设施园艺技术重点实验室;扬州大学农学院,江苏,扬州,225009
摘    要:通过温室水培试验,研究1.0×10-4mol/LZn2 胁迫及解除胁迫对番茄(Lycopersicom esculentum Mill.)幼苗根系中抗氧化系统的动态变化.结果表明,胁迫条件下,番茄根系丙二醛(MDA)和蛋白质含量增加;超氧化物歧化酶(SOD,EC1.15.1.1),过氧化氢酶(CAT,EC1.11.1.6)和谷胱甘肽过氧化物酶(GPX,EC1.11.1.9)活性上升,而过氧化物酶(POD,EC1.11.1.7)活性下降;AsA-GSH循环代谢受影响;抗坏血酸过氧化酶(APX,EC1.11.1.11)活性在胁迫d1、d3上升,随后下降,而谷胱甘肽还原酶(GR,EC1.6.4.2)活性在胁迫1~5d时下降,随后上升;抗坏血酸(AsA)和谷胱甘肽(GSH)含量上升.解除胁迫后,根系中MDA和蛋白质含量随时间延长逐渐减少,接近对照;POD和APX活性增加,而SOD、CAT和GR活性及GSH含量先升高,随后减少;GPX活性和AsA含量却降低;恢复后根系中抗氧化物水平仍然高于对照.依据实验结果,文中讨论了胁迫及恢复过程中植物AsA-GSH循环代谢的作用.图3参16

关 键 词:番茄(Lycopersicom  esculentum  Mill.)  Zn2    丙二醛  抗氧化系统  AsA-GSHcycle循环
收稿时间:2004-11-16
修稿时间:2005-02-22

DYNAMIC CHANGES IN ANTIOXIDATIVE SYSTEMS IN ROOTS OF TOMATO (LYCOPERSICOM ESCULENTUM MILL.) SEEDLING UNDER ZINC STRESS AND RECOVERY
DING Haidong,ZHU Weimin,YANG Shaojun,YANG Xiaofeng.DYNAMIC CHANGES IN ANTIOXIDATIVE SYSTEMS IN ROOTS OF TOMATO (LYCOPERSICOM ESCULENTUM MILL.) SEEDLING UNDER ZINC STRESS AND RECOVERY[J].Chinese Journal of Applied and Environmental Biology,2005,11(5):531-535.
Authors:DING Haidong  ZHU Weimin  YANG Shaojun  YANG Xiaofeng
Abstract:A solution culture experiment was carried out in greenhouse to study the dynamic changes in antioxidative systems in roots of tomato seedlings (Lycopersicom esculentum Mill.) under zinc stress and recovery. The results showed that under stress malonaldehyde (MDA) and protein contents increased, and the activities of superoxide dismutase (SOD, EC 1.15.1.1), catlase (CAT, EC 1.11.1.6) and glutathione peroxidase (GPX, EC 1.11.1.9) increased, while guaiacol peroxidase (POD, EC 1.11.1.7) activity declined. AsA-GSH cycle was affected. Ascorbate peroxidase (APX, EC 1.11.1.11) activity rose in the first three days and decreased then, and glutathione reductase (GR, EC 1.6.4.2) activity decreased in the first five days and increased then, the contents of ascrobate (AsA) and glutathione (GSH) increased as well. After recovery, MDA and protein contents declined to control level. The activities of POD and APX increased, while the activities of SOD, CAT and GR and GSH content increased in the beginning of recovery, and then decreased. GPX activity and AsA content in roots duratively declined. After recovery the activities and contents remained higher than those of controls. According to the results of the experiment, the role of AsA-GSH cycle during the stressed and recovering period was discussed in this paper. Fig 3, Ref 16
Keywords:tomato (Lycopersicom esculentum Mill  )  zinc  malonaldehyde  antioxidative systems  AsA-GSH cycle
本文献已被 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号