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| 绿色荧光蛋白基因标记内生枯草芽孢杆菌 | |
| 引用本文： | 范晓静,邱思鑫,吴小平,洪永聪,蔡学清,胡方平.绿色荧光蛋白基因标记内生枯草芽孢杆菌[J].应用与环境生物学报,2007,13(4):530-534. |
| 作者姓名： | 范晓静 邱思鑫 吴小平 洪永聪 蔡学清 胡方平 |
| 作者单位： | 1. 福建农林大学生物农药与化学生物学教育部重点实验室;福建农林大学生命科学学院,福州,350002 2. 福建省农科院蔬菜研究中心,福州,350013 3. 福建农林大学生命科学学院,福州,350002 4. 福建农林大学植物保护学院,福州,350002 |
| 基金项目： | 国家自然科学基金;福建省科技计划 |
| 摘 要： | 用枯草芽孢杆菌168菌株rpsD基因的启动子替换质粒pGFP4412中蜡状芽孢杆菌4412启动子,从而构建了能在枯草芽孢杆菌中表达绿色荧光蛋白基因gfpmut3a的载体pS4GFP,将其导入具有内生、防病、促生作用的野生型枯草芽孢杆菌BS-2菌株中,筛选获得遗传稳定性好且具有良好发光表型的标记菌株BS-2-gfp.该标记菌株在小白菜体内的定殖研究结果表明,该菌株能够在小白菜根际及根、茎、叶内定殖和传导,接菌50d后仍能在其体内分离到标记菌株.图5参17 |
| 关 键 词： | 枯草芽孢杆菌 绿色荧光蛋白(GFP) 表达 植物内生细菌 |
| 收稿时间： | 2006-03-13 |
| 修稿时间： | 2006-06-19 |
Endophytic Bacillus subtilis Strain BS-2 Labeled with Green Fluorescent Protein Gene |
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| FAN Xiaojing,QIU Sixin,WU Xiaoping,HONG Yongcong,CAI Xueqing,HU Fangping.Endophytic Bacillus subtilis Strain BS-2 Labeled with Green Fluorescent Protein Gene[J].Chinese Journal of Applied and Environmental Biology,2007,13(4):530-534. | |
| Authors: | FAN Xiaojing QIU Sixin WU Xiaoping HONG Yongcong CAI Xueqing HU Fangping |
| Institution: | 1. Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 .College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China ; 3. Vegetable Research Centre, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;4.College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
| Abstract: | Promoter 4412 of plasmid pGFP4412 was replaced by rpsD promoter of Bacillus subtilis strain 168 to obtain a new vector pS4GFP in which the gfp gene could strongly express under the control of rpsD promoter. Plasmid pS4GFP was transformed into the endophytic B. subtilis strain BS-2, and a fine gfp-labeled strain BS-2-gfp was obtained. The tests showed the strain could keep promoting plant growth and inhibiting plant pathogens. The strain also could appear bright green under blue light and the recombined plasmid was stable in the bacterial cells. The colonizing inside of cabbages with the strain showed that the bacteria could adhere to the surface of roots, and could be colonized in roots, stems and leaves. After inoculation for 50 days, the labeled bacteria could still be isolated from the cabbages. Fig 5, Ref 17 |
| Keywords: | Bacillus subtilis green fluorescent protein (GFP) expression endophytic bacterium |
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