| 一株菲降解菌的特性及相关降解基因的克隆 |
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| 引用本文： | 何丽娟,李正华,洪青,李顺鹏.一株菲降解菌的特性及相关降解基因的克隆[J].应用与环境生物学报,2009,15(5). |
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| 作者姓名： | 何丽娟 李正华 洪青 李顺鹏 |
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| 作者单位： | 1. 南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,南京,210095
2. 江苏省宜兴市前程生物有限公司,宜兴,214253 |
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| 基金项目： | 国家"863"计划项目，科技部自然科技资源平台项目 |
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| 摘 要： | 从石油污染土壤中分离到一株菲降解菌2F5-2.根据该菌株生理生化特征和16S rDNA序列相似性分析,将其初步鉴定为鞘氨醇杆菌属(Sphingobium sp.).该菌株在10 h内对100 mg/L的菲的降解率为100%.降解菲的最适温度为30℃,最适pH为7.对降解途径的初步研究显示,该菌株通过水杨酸途径降解菲.克隆了编码芳香烃双加氧酶α亚基的基因phdA,它与菌株Sphingomonas sp.P2、Sphingobium yanoikuyae B1、Sphingomonas sp.ZP1中phdA的同源性分别为97.9%、98%和100%,表明该基因具有保守性.图6参16
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| 关 键 词： | 菲 生物降解 降解途径 phdA基因 |
Characterization of A Phenanthrene-degrading Strain and Cloning of Degradation-related Gene |
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| HE Lijuan,LI Zhenghua,HONG Qing,LI Shunpeng.Characterization of A Phenanthrene-degrading Strain and Cloning of Degradation-related Gene[J].Chinese Journal of Applied and Environmental Biology,2009,15(5). |
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| Authors: | HE Lijuan LI Zhenghua HONG Qing LI Shunpeng |
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| Abstract: | A bacterial strain 2F5-2 capable of degrading phenanthrene was isolated from petroleum-contaminated soil. It was preliminarily identified as Sphingobium sp. according to its physiological & biochemical characteristics and the analysis of its 16S rRNA gene sequence. Strain 2F5-2 could degrade 100% of 100 mg/L phenanthrene within 10 h .The optimal pH and temperature for the degradation were 7 and 30 ℃, respectively. The analysis of its phenanthrene-degrading pathway revealed that strain 2F5-2 degraded phenanthrene via salicylate pathway. The aromatic-ring-hydroxylating dioxygenases α-subunit encoding gene phdA was cloned and analyzed. It had 97.9%, 98%, 100% identity to the phdA gene from Sphingomonas sp. P2, Sphingobium yanoikuyae B1 and Sphingomonas sp. ZP1, respectively, indicating the conservation of this gene. Fig 6, Ref 16 |
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| Keywords: | phenanthrene biodegradation degrading pathway phdA gene |
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