油松毛虫感染白僵菌后超氧化物歧化酶和过氧化氢酶的变化

采用分光光度法测定了3~5龄油松毛虫(DendrolimustabulaeformisTsaietLiu)幼虫被白僵菌(Beauveriabassi ana)感染后保护酶的活性.结果表明: 3~5龄油松毛虫被白僵菌感染后1 ~8d,体内超氧化物歧化酶(SOD)活性显著高于同期未感染的幼虫,并呈现出先升高后下降的变化趋势,而同期未感染的幼虫变化不大;过氧化氢酶(CAT)的活性也呈现出先升后降的趋势.利用聚丙烯酰胺凝胶电泳技术,分析了感染后1~8d幼虫体内SOD同工酶与CAT同工酶的变化.结果显示:SOD同工酶带宽与酶带亮暗变化显著;CAT同工酶酶谱有所差异,酶带数由感染初期的一条变为后期的两条带,而且带宽值与酶带亮暗变化显著. 图3表2参16

CHANGES OF SUPEROXIDE DISMUTASE AND CATALASE IN DENDROLIMUS TABULAEFORMIS INFECTED BY BEAUVERIA BASSIANA

The method of spectrophotometry was used to analyze the activities of endogenous enzymes of protective system in Dendrolimus tabulaeformis Tsai et Liu parasitized by Beauveria bassiana . The result showed that when larvae in their 3 rd and 4 th instars were infected by B. bassiana the superoxide dismutase (SOD) activities were significantly higher than those of the non-infected ones. The changing trend appeared to rise at the first phase and then drop at the last phase. The catalase (CAT) activities showed the same trend as SOD. By using polyacrylamide gel electrophoresis(PAGE)techniques, SOD and CAT in the body were successively determined from the first to the eighth day after the larvae were infected by the fungi. The results showed that some differences in SOD appeared in band width and staining intensity. Meanwhile the CAT bands had slight differences in spectrum of enzyme. The band number changed from one in the early days into two in the later ones. At the same time the band widths and staining intensities indicated significant differences. Fig 3, Tab 2, Ref 16