苏云金芽胞杆菌YBT-1520中SigL及其增强子结合蛋白的结构与功能分析

为揭示SigL及其增强子结合蛋白(EBPs)在苏云金芽胞杆菌(Bt)中的调控功能,在全基因组测序的基础上,采用生物信息学方法,对YBT-1520菌株的SigL及其EBPs的结构和功能进行深入分析.结果表明,YBT-1520基因组中存在1个SigL和6个EBPs,而且EBPs在结构上具有丰富的多样性,包含了EBPs的所有可能的结构域组织类型.SigL所调控的基因涉及11个假定的COG代谢途径,其中包括能量代谢、氨基酸代谢、翻译与细胞周期等.根据EBPs在基因组的位置推测,YBT-1520的EBPs参与γ-氨基丁酸代谢途径、精氨酸代谢途径、支链脂肪酸降解途径、多糖分解代谢等代谢途径的调控.本研究将为揭示Bt杀虫晶体蛋白大量表达的调控机制提供新的思路.

Structure and Function Analysis of SigL and Its Enhancer-binding Proteins in Bacillus thuringiensis Strain YBT-1520

To reveal the regulatory functions of SigL and its enhancer-binding proteins(EBPs) in Bacillus thuringiensis,the structure and function of SigL and its EBPs of YBT-1520 strain were in depth analyzed through bioinformatics methods after the whole genome sequencing.The results indicated that there were one and six coding genes for SigL and EBPs in YBT-1520 genome,respectively.Furthermore,the EBPs displayed rich structural diversity and possessed all organization types of EBPs domains.The genes regulated by SigL should be related with 11 putative COG metabolism pathways,including energy metabolism,amino acid metabolism,translation and cell cycle.According to the location of EBPs in YBT-1520 genome,some EBPs were predicted to regulate the metabolism pathways of γ-aminobutyric acid and arginine,and the degradation of branched chain fatty acid and polysaccharide.This study will provide a new research direction on the regulatory mechanisms for high expression of insecticidal crystal proteins.