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| 具双T-DNA区的大麦黄矮病毒复制酶基因RNAi植物表达载体的构建(英文) | |
| 引用本文： | 孙文瑜,陈静,王磊,付体华.具双T-DNA区的大麦黄矮病毒复制酶基因RNAi植物表达载体的构建(英文)[J].应用与环境生物学报,2008,14(6). |
| 作者姓名： | 孙文瑜 陈静 王磊 付体华 |
| 作者单位： | 1. 中国科学院成都生物研究所,成都,610041;四川农业大学农学院,雅安,625014 2. 中国科学院成都生物研究所,成都,610041 3. 中国农业科学院生物技术研究所,北京,100081 4. 四川农业大学农学院,雅安,625014 |
| 基金项目： | Supported by the National Natural Science Foundation of China (No. 30871527); the Knowledge Innovation Project of the Chinese Academy of Science |
| 摘 要： | RNAi系双链RNA诱导同源mRNA降解,导致特定基因表达沉默的一种现象.RNAi技术是通过基因工程防治植物病毒病的最佳途径.由大麦黄矮病毒(Barely yellow dwarfvirus,简称BYDV)引起的禾谷类黄矮病发病面积和危害程度在我国呈加重趋势.BYDV-GAV是当前流行的大麦黄矮病毒株系,本文针对BYDV-GAV复制酶基因序列,构建能在细胞内转录形成发卡RNA双链结构的表达盒,并将其置于中间载体pVec8-2b的T-DNA区,pVec8-2b的另一T-DNA区含有hpt选择报告基因表达盒.具双T-DNA区的病毒复制酶基因发卡RNA高效表达载体已导入根癌农杆菌,可用于诱导植物RNAi,创制无选择标记基因的抗大麦黄矮病转基因作物. |
| 关 键 词： | 大麦黄矮病毒BYDV 双T-DNA区载体 无选择标记基因植物 |
Construction of Twin T-DNA Vector Encoding Hairpin RNA Targeting the Replicase Gene of Barley Yellow Dwarf Virus |
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| SUN Wenyu,CHEN Jing,WANG Lei,FU Tihua.Construction of Twin T-DNA Vector Encoding Hairpin RNA Targeting the Replicase Gene of Barley Yellow Dwarf Virus[J].Chinese Journal of Applied and Environmental Biology,2008,14(6). | |
| Authors: | SUN Wenyu CHEN Jing WANG Lei FU Tihua |
| Abstract: | RNA interference (RNAi) technology has become the most effective approach for genetically engineering virus resistance in plants. Here, we constructed a hairpin RNA (hpRNA)-expressing RNAi vector targeting the replicase gene of a Chinese isolate of barley yellow dwarf virus (BYDV), BYDV-GAV. This construct was comprised of two tandem T-DNAs (twin T-DNAs), one of which contained the hygromycin resistance gene hpt as the selectable marker, and the other contained the expression cassette encoding hpRNA of the ... |
| Keywords: | RNAi barley yellow dwarfvirus(BYDV) RNAi twin T-DNAs vector marker-free plant |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |