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| 玉米种子的DNA指纹计算机化鉴定 | |
| 引用本文： | 陈一华,张超良,王泽立,贾建航,孙致良,金德敏,王斌.玉米种子的DNA指纹计算机化鉴定[J].应用与环境生物学报,2000,6(3):223-226. |
| 作者姓名： | 陈一华 张超良 王泽立 贾建航 孙致良 金德敏 王斌 |
| 作者单位： | 1. 中国科学院遗传研究所,北京,100101 2. 山东农业大学,泰安,271018 3. 莱阳农学院,莱阳,265200 |
| 摘 要： | 对１２个优良玉米自效系的ＤＮＡ进行了ＲＡＰＤ分析，共筛选了２５０个Ｏｐｅｒｏｎ引物，其中１２个引物共扩增出５９个多态性产物，根据这５９个多态性ＲＡＰＤ产物，对１２个自交系进行了聚类分析，把它们分为３个群，结果与根据系谱法的聚类结果一致。经过优选，用ＯＰＮ－１１扩增出的１１种ＤＮＡ带，建立了这１２个自交系的ＲＡＰＤ－ＤＮＡ指纹图谱。在该图谱呼弱个自交系都具有特异的ＤＮＡ指纹，可以与其它自效纱相区别。 |
| 关 键 词： | 计算机应用 DNA5指纹 种子鉴定 玉米 RAPD |
COMPUTERIZED IDENTIFICATION OF DNA FINGERPRINTING OF MAIZE SEED |
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| CHEN Yihua,ZHANG Chaoliang,WANG Zeli,JIA Jianhang,SUN Zhiliang,JIN Demin,WANG Bin.COMPUTERIZED IDENTIFICATION OF DNA FINGERPRINTING OF MAIZE SEED[J].Chinese Journal of Applied and Environmental Biology,2000,6(3):223-226. | |
| Authors: | CHEN Yihua ZHANG Chaoliang WANG Zeli JIA Jianhang SUN Zhiliang JIN Demin WANG Bin |
| Abstract: | 12 elite maize inbred lines were examined by means of RAPD analysis. Among the 250 Operon primers screened, 12 gave reproducible, polymorphic DNA amplification patterns. 11 bands amplified by primer OPN-11 were used in the development of RAPD-DNA fingerprinting of the 12 inbred lines. Each inbred line has its unique fingerprinting and can be easily distinguished from each other. The RAPD results were scored based on the presence or absence of each band and then converted to computer language with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of each band, respectively. The RAPD-DNA fingerprinting analysis developed by this research can be used in practical seed identification of the 12 elite maize inbred lines, for both true and false test as well as purity determination. Fig 4, Tab 1, Ref 6 |
| Keywords: | computer utilization DNA fingerprinting seed identification maize RAPD |
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