| 拟南芥硫酯酶基因(atfata)在大肠杆菌中的表达及其对游离脂肪酸合成的影响 |
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| 引用本文： | 程涛,杨建明,刘辉,张英伟,宋秋华,咸漠.拟南芥硫酯酶基因(atfata)在大肠杆菌中的表达及其对游离脂肪酸合成的影响[J].应用与环境生物学报,2011(4):568-571. |
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| 作者姓名： | 程涛 杨建明 刘辉 张英伟 宋秋华 咸漠 |
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| 作者单位： | 江西理工大学材料与化学工程学院;中国科学院青岛生物能源与过程研究所;旭阳控股有限公司; |
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| 基金项目： | 中国科学院知识创新工程重要方向项目(No.KGCXZ-YW-801)资助~~ |
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| 摘 要： | 硫酯酶催化脂酰ACP(酰基载体蛋白)水解生成游离脂肪酸和ACP载体蛋白,能够解除脂肪酸合成与磷脂合成的偶联,是获得游离脂肪酸的关键基因.将拟南芥的硫酯酶基因atfata克隆到原核表达载体pET30a上,在大肠杆菌BL21(DE3)中成功表达了His-tag融合蛋白,经SDS-PAGE检测获得特异性表达条带.对该重组菌株进行摇瓶发酵实验,GC-MS检测表明其与对照菌株脂肪酸组成并未发生变化;但其脂肪酸产量达到232.06 mg/L,比对照菌株提高了70%;同时胞外游离脂肪酸产量达到了33 mg/L,占总脂肪酸含量的15%,是野生菌株胞外脂肪酸产量的7倍.图3表2参17
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| 关 键 词： | 拟南芥 硫脂酶 工程大肠杆菌 胞外游离脂肪酸 |
Expression of Arabidopsis thaliana Thioesterase Gene(atfata) in Escherichia coli and Its Influence on Biosynthesis of Free Fatty Acid |
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| CHENG Tao,YANG Jianming,LIU Hui,ZHANG Yingwei,SONG Qiuhua & XIAN Mo.Expression of Arabidopsis thaliana Thioesterase Gene(atfata) in Escherichia coli and Its Influence on Biosynthesis of Free Fatty Acid[J].Chinese Journal of Applied and Environmental Biology,2011(4):568-571. |
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| Authors: | CHENG Tao YANG Jianming LIU Hui ZHANG Yingwei SONG Qiuhua & XIAN Mo |
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| Institution: | CHENG Tao1,YANG Jianming2,LIU Hui2,ZHANG Yingwei3,SONG Qiuhua1 & XIAN Mo2(1College of Material Science and Chemical Engineering,Jiangxi University of Science and Technology,Ganzhou 341000,China)(2Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,China)(3Xuyang Holding Ltd.,Beijing 100070,China) |
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| Abstract: | Acyl-acyl carrier protein(ACP) thioesterase catalyzes the hydrolysis of acyl-ACP to fatty acids and ACP.This reaction removes the coupling between fatty acid biosynthesis and phospholipid biosynthesis,which is a key to obtain free fatty acids.In this study,a thioesterase gene from Arabidopsis thaliana(atfata) was cloned into pET30a vector and heterologously expressed in Escherichia coli BL21(DE3).SDS-PAGE analysis revealed a special band corresponding to the fusion protein.Under shake-flask conditions,this ... |
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| Keywords: | Arabidopsis thaliana thioesterase engineered Escherichia coli extracellular free fatty acid |
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