| 太湖和巢湖夏季蓝藻水华期间产毒微囊藻和非产毒微囊藻种群丰度的空间分布 |
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| 引用本文： | 李大命,孔繁翔,张民,阳振.太湖和巢湖夏季蓝藻水华期间产毒微囊藻和非产毒微囊藻种群丰度的空间分布[J].应用与环境生物学报,2011(4):480-485. |
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| 作者姓名： | 李大命 孔繁翔 张民 阳振 |
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| 作者单位： | 中国科学院南京地理与湖泊研究所;中国科学院研究生院; |
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| 基金项目： | 国家“973”计划项目(No.2008CB418000); 中国科学院南京地理与湖泊研究所所长基金(No.07SL021001)资助~~ |
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| 摘 要： | 应用荧光定量PCR技术对蓝藻水华暴发期间太湖和巢湖水体中产毒微囊藻和总微囊藻种群丰度的空间分布进行了研究.分别以微囊藻毒素合成基因基因家族成员mcyD和小核糖体16S rDNA序列构建定量PCR标准曲线,研究产毒微囊藻和总微囊藻种群丰度.结果表明:太湖和巢湖微囊藻种群由产毒微囊藻和非产毒微囊藻组成;蓝藻水华暴发期间,微囊藻种群组成及其丰度分布具有明显的空间差异性:太湖产毒微囊藻种群丰度为9.89×104～4.51×106 copies mL-1,产毒微囊藻占总微囊藻种群的比例为20.5%～38.1%;巢湖产毒微囊藻种群丰度为4.12×104～5.44×106 copies mL-1,其占总微囊藻种群的比例为8.4%～96.6%.总的来说,富营养化严重的湖区总微囊藻和产毒微囊藻种群丰度较高,产毒微囊藻占总微囊藻种群的比例也较高.图7表3参29
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| 关 键 词： | 微囊藻 产毒微囊藻 种群丰度 荧光定量PCR 太湖 巢湖 蓝藻水华 |
Spatial Changes in Abundance of Microcystin-producing and Non-microcystin Producing Microcystis Populations in the Taihu Lake and the Chaohu Lake During Cyanobacterial Bloom Period |
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| LI Daming,KONG Fanxiang,ZHANG Min & YANG Zhen.Spatial Changes in Abundance of Microcystin-producing and Non-microcystin Producing Microcystis Populations in the Taihu Lake and the Chaohu Lake During Cyanobacterial Bloom Period[J].Chinese Journal of Applied and Environmental Biology,2011(4):480-485. |
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| Authors: | LI Daming KONG Fanxiang ZHANG Min & YANG Zhen |
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| Institution: | LI Daming1,2,KONG Fanxiang1,ZHANG Min1 & YANG Zhen1(1Nanjing Institue of Geography and Limnology,Chinese Academy of Sciences,Nanjing 210008,China)(2Graduate University of Chinese Academy of Sciences,Beijing 100049,China) |
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| Abstract: | The spatial changes in abundances of microcystin-producing and total Microcystis populations in the Taihu Lake and the Chaohu Lake during water bloom were investigated using quantitative real-time PCR(qPCR) techniques.Primer sets based on the small subunit ribosomal RNA gene(16S rRNA) and the microcystin synthetase gene(mcyD) were used to target the total Microcystis population and the potential microcystin-producing subpopulation,respectively.The results showed that Microcystis existed as a mixed populatio... |
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| Keywords: | Microcystis microcystin-producing Microcystis population abundance quantitative real-time PCR Taihu Lake Chaohu Lake cyanobacterial bloom |
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