| 产油脂微藻的分离鉴定及培养条件优化 |
| |
| 引用本文： | 于荣清,刘义,田思琪,杨云喜,李伟,曹毅,乔代蓉.产油脂微藻的分离鉴定及培养条件优化[J].应用与环境生物学报,2011(6):897-900. |
| |
| 作者姓名： | 于荣清 刘义 田思琪 杨云喜 李伟 曹毅 乔代蓉 |
| |
| 作者单位： | 四川大学生命科学学院微生物与代谢工程四川省重点实验室; |
| |
| 基金项目： | 四川省科技支撑计划(Nos.2008GZ0021,2008GZ0200); 国家科技基础工作专项重点项目(No.2008FY11400-4-1)~~ |
| |
| 摘 要： | 为促进自养高产油微藻的工业化应用,从青海多种生境中分离到34株产油微藻,以筛选获得产油脂最高的青3-2-2株为出发株,18S rRNA分析表明其与栅列藻属(Scenedesmus sp.)同源性达到99%以上,确定该株属于栅列藻属.研究氮源、培养时间、培养温度、初始pH值等培养条件对微藻生物量及油脂含量的影响,优化培养条件为:硝酸钠为氮源,以10%(V/V)接种量接种于普通SE培养基,培养温度为25℃,初始pH 7.0,培养周期为20 d,可以获得较高的油脂产率,比优化前的产率增加了近70%.
|
| 关 键 词： | 微藻 油脂 栅列藻属 菌株分离鉴定 培养条件优化 |
Isolation and Identification of Oil Microalgae and Optimization of Its Culture Conditions |
| |
| YU Rongqing,LIU Yi,TIAN Siqi,YANG Yunxi,LI Wei,CAO Yi & QIAO Dairong.Isolation and Identification of Oil Microalgae and Optimization of Its Culture Conditions[J].Chinese Journal of Applied and Environmental Biology,2011(6):897-900. |
| |
| Authors: | YU Rongqing LIU Yi TIAN Siqi YANG Yunxi LI Wei CAO Yi & QIAO Dairong |
| |
| Institution: | YU Rongqing,LIU Yi,TIAN Siqi,YANG Yunxi,LI Wei,CAO Yi & QIAO Dairong(Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province,College of Life Sciences,Sichuan University,Chengdu 610065,China) |
| |
| Abstract: | Thirty-four algae strains were isolated from diverse environments in Qinghai,China.All of them were identified as oil microalgae,and the most oil-rich strain named Qing 3-2-2 was used as the test strain.The homology of 18S rRNA fragments between Qing 3-2-2 and the strains in Scenedesmus genus was high,up to 99% identical.Thus,the strain was identified to belong to Scenedesmus genus.Effects of culture factors,such as N source,incubation time,culture temperature and initial pH value,on biomass production and ... |
| |
| Keywords: | microalgae lipid Scenedesmus sp strain isolation and identification optimization of culture condition |
| 本文献已被 CNKI 等数据库收录！ |
|
