| 一株中度嗜盐淀粉酶产生菌的分离鉴定及酶活测定 |
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| 引用本文： | 刘洋,栗敏,郭泽经,何艳,冯治翔,宋昭彬,田永强.一株中度嗜盐淀粉酶产生菌的分离鉴定及酶活测定[J].应用与环境生物学报,2011(6):883-886. |
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| 作者姓名： | 刘洋 栗敏 郭泽经 何艳 冯治翔 宋昭彬 田永强 |
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| 作者单位： | 四川大学生命科学学院;四川大学化学工程学院;中国医药集团四川抗菌素工业研究所; |
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| 摘 要： | 为开发极端环境工业用酶,从新疆盐碱土微生物中分离得到一株中度嗜盐高产淀粉酶活性菌株H3,其能耐受30%盐浓度和pH 11的极端环境.通过形态特征、生理生化实验及16S rRNA基因序列分析,确定H3属于Gracilibacillus属.该菌株能在盐浓度为0~30%的培养基上生长,最适生长盐浓度为5%~10%,最适pH值为8.5.在最适生长盐浓度、pH条件下,其淀粉酶活性可达到4 830个活力单位,可用于高盐高碱环境下淀粉的水解.
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| 关 键 词： | 嗜盐细菌 菌株鉴定 Gracilibacillus sp. 系统进化 淀粉酶 酶活测定 高盐碱环境 |
Isolation and Identification of a Moderately Halophilic Amylase-producing Strain and Determination of Amylase Activity |
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| LIU Yang,LI Min,GUO Zejing,HE Yan,FENG Zhixiang,SONG Zhaobin & TIAN Yongqiang.Isolation and Identification of a Moderately Halophilic Amylase-producing Strain and Determination of Amylase Activity[J].Chinese Journal of Applied and Environmental Biology,2011(6):883-886. |
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| Authors: | LIU Yang LI Min GUO Zejing HE Yan FENG Zhixiang SONG Zhaobin & TIAN Yongqiang |
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| Institution: | LIU Yang1,LI Min2,GUO Zejing3,HE Yan2,FENG Zhixiang3,SONG Zhaobin1 & TIAN Yongqiang2,3(1College of Life Sciences,Sichuan University,Chengdu 610065,China)(2College of Chemical Engineering,China)(3Sichuan Industrial Institute of Antibiotics,China National Pharmaceutical Group Corporation,China) |
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| Abstract: | A high-yield amylase strain,named as H3,was isolated from saline-alkali soil in Xinjiang,China.H3 could grow under the condition with 30% NaCl concentration and pH 11.Based on the results of morphological,physiological and biochemical experiments,and analysis of 16S rRNA sequence,this strain was identified as Gracilibacillus sp.It could grow on the media containing 0~30% NaCl.The optimum NaCl concentration for H3 growth was 5%~10% and the optimum pH was 8.5.The highest amylase activity was 4 830 units under... |
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| Keywords: | halophilic bacterium strain identification Gracilibacillus sp phylogenetic analysis amylase enzyme activity determination high salt and alkaline condition |
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