| 矮牵牛花香基因BSMT3的cDNA克隆与表达特征分析 |
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| 引用本文： | 喻麟,淳泽,张海英,杨涛,王立洪,刘世贵,龙章富.矮牵牛花香基因BSMT3的cDNA克隆与表达特征分析[J].应用与环境生物学报,2007,13(2):176-179. |
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| 作者姓名： | 喻麟 淳泽 张海英 杨涛 王立洪 刘世贵 龙章富 |
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| 作者单位： | 1. 四川大学生命科学学院/生物资源与生态环境教育部重点实验室,成都,610064
2. 中国科学院成都生物研究所,成都,610041 |
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| 摘 要： | 以矮牵牛花瓣总RNA为模板,RT-PCR方法克隆得到其BSMTcDNA,全长1100bp,编码357aa,与已有报道phBSMT1(GenBank No.AA0501)和phBSMT2(AAO45013)的同源性分别达到97.76%和98.6%,该序列在Gen-Bank登录号为DQ494491,命名为phBSMT3.构建的BSMT3 cDNA的原核表达质粒pGBSMT转化BL21(DE3)E.coli,获得的重组菌株经0.01mol/LIPTG诱导后得到正确表达的GST-BSMT融合蛋白带,以纯化的重组表达蛋白免疫家兔获得其兔抗免疫血清.免疫组化研究结果证实,矮牵牛花香基因BSMT在花瓣、花管中的表达量较其它组织的表达量高.图5参24
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| 关 键 词： | 矮牵牛 克隆 表达 |
| 收稿时间： | 2006-10-10 |
| 修稿时间： | 2006-12-31 |
cDNA Cloning and Expression Characteristics of Floral Scent Gene BSMT3 of Petunia hybrida |
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| YU Lin,CHUN Ze,ZHANG Haiying,YANG Tao,WANG Lihong,LIU Shigui,LONG Zhangfu.cDNA Cloning and Expression Characteristics of Floral Scent Gene BSMT3 of Petunia hybrida[J].Chinese Journal of Applied and Environmental Biology,2007,13(2):176-179. |
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| Authors: | YU Lin CHUN Ze ZHANG Haiying YANG Tao WANG Lihong LIU Shigui LONG Zhangfu |
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| Institution: | 1. Key Laboratory of Bio-Resource and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China;2.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China |
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| Abstract: | A new cDNA(GenBank DQ494491)was cloned from Petunia hybrida by RT-PCR using the total RNA of its petals as template.The sequence was 1 100 bp in length and encoded a mature peptide with 357 amino acids.This cDNA named as phBSMT3 shared 97.76% and 98.6% homologies to phBSMT1(AAO4501)and phBSMT2(AAO45013)reported for P.hybrida.The recombinant expression strain,pGBSMT/BL21,was constructed by sub-cloning the cDNA under the promoter of vector pGEX4T-1 and the identified recombinant plasmid pGBSMT was transformed into BL21(DE3)E.coli.This strain was expressed by the induction of 0.01mol/L IPTG,and the fusion expression product GST-BSMT was purified and used as the antigen to immunize rabbits in order to prepare target polyclonal antibody.The results of immuno-histochemical analyses showed that the BSMT3 gene was expressed largely in the petals and tubes of P.hybrida when compared with other tissues,such as leaves,stems and calyces.Fig 5,Ref 24 |
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| Keywords: | BSMT cDNA |
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