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籼粳杂种不育突变体91FS育性特征和RAPD及RFLP分析
引用本文:钟蓉,董微,肖邡明,袁水泉,吴伯骥.籼粳杂种不育突变体91FS育性特征和RAPD及RFLP分析[J].应用与环境生物学报,2000,6(5):423-427.
作者姓名:钟蓉  董微  肖邡明  袁水泉  吴伯骥
作者单位:中国科学院成都生物研究所成都 610041
基金项目:中国科学院“九五”基础性研究重点项目(KJ982-JI-624)、国家自然科学基金资助项目(No:39570468)、国家计委科技攻关课题(No:85-722-25-01)、中国科学院成都地奥科学基金(DASF)和四川省重点科学技术课题资助项目
摘    要:91FS是一个稳定遗传的籼粳杂种水稻新型不育突变体,育性特征表现为,雌雄配子和受精过程正常,约25%的受精卵发育成胚,约75%的受精卵终止发育,与91FS群体约25%的结实率吻合,而且六代保持不变;91FS的RAPD和RFLP的谱带六代保持稳定和一致,与91FS的杂种性状和育性表达的稳定性也是吻合的;91FS与籼粳杂种可育材料91FM和以91FS为父本的杂交F

关 键 词:籼粳杂种  不育突变体  育性  RAPD  RFLP
修稿时间:2000年1月27日

FERTILITY CHARACRERISTIC AND RAPD/RFLP ANALYSIS OF A NEW HERITABLE INDICA-JAPONICA RICE HYBRID STERILE MUTANT 91FS
ZHONG Rong,DONG Wei,Xiao Fangming,YUAN Shuiquan,WU Boji.FERTILITY CHARACRERISTIC AND RAPD/RFLP ANALYSIS OF A NEW HERITABLE INDICA-JAPONICA RICE HYBRID STERILE MUTANT 91FS[J].Chinese Journal of Applied and Environmental Biology,2000,6(5):423-427.
Authors:ZHONG Rong  DONG Wei  Xiao Fangming  YUAN Shuiquan  WU Boji
Abstract:The fertility characteristic of 91FS, a new heritable Indica-japonica rice hybrid sterile mutant, displayed its normal female/male gametes and fertilization process. About 25% of the fertilized eggs could develop into mature embryos while about 75% of the fertilized eggs stopped further developing, which coincided with the population seed percentage of 91FS. In addition, this feature kept constant within six generations. The stability and consistency of 91FS RFLP bands coincided with the stability of hybrid characteristic and fertility expression. The RAPD results of 91FS, 91FM(a fertile Indica-japonica rice hybrid) and F 1(D297AX91FS) showed that there were quite different kinds of DNA polymorphism among 91FS and the other two controls. 91FS also displayed some new special DNA bands and absent bands. The polymorphism, identity and specificity of 91FS are heritable. Fig 4, Tab 1, Ref 15
Keywords:Indica  japonica rice hybrid  sterile mutant  fertility  RAPD  RFLP
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