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Fish as an indicator of aquatic environment: Multielemental neutron activation analysis of nutrient and pollutant elements in fish from Indian coastal areas
Authors:A N Garg  V V S Ramakrishna
Institution:1. Department of Chemistry , Indian Institute of Technology , Roorkee – 247667, India agargfcy@iitr.ernet.in;3. Nagpur University , Nagpur – 440010, India
Abstract:Fish is the main food in coastal areas and its analysis for toxic metals has been used as an indicator of the pollution status of the aquatic environment. Several different fish species from the three Indian coasts, Visakhapatnam in the east, Mumbai in the west and Mangalore in the south west and also inland freshwater fish from Nagpur in the central region for comparison were analyzed for up to 20 elements (As, Au, Ba, Br, Cl, Co, Cr, Cu, Fe, Hg, K, Mn, Na, P, Rb, Sb, Sc, Se, Sr, Zn) by instrumental neutron activation analysis (INAA). Dried fish samples in powdered form were irradiated with thermal neutrons in a nuclear reactor followed by high resolution gamma ray spectrometry at different intervals. Several Reference Materials (RMs) of biological origin were analyzed for quality assurance and data validation. Elemental contents in different fish species vary in a wide range depending on the species, its size, location, and aquatic environment. Fish from Mangalore showed highest mean contents of Cr (14.8?±?29.9?µg?g?1), Cu (1005?±?643?µg?g?1) and Sb (849?±?888?ng?g?1) whereas those from Mumbai exhibited highest Hg (2066?±?2146?ng?g?1) and P (20.3?±?4.63?µg?g?1) but lowest Cu (6.30?±?3.10?µg?g?1) contents. Fish from all the regions showed significant amounts of nutrient elements such as Na, K, P, Mn, Fe, Se and Zn as well as some pollutant elements (Br, Cr, Sb, Hg). No regularity in variation of elemental contents with the size was observed. Prawn (Panaeus latisulcatus), a popular fish species from four different regions showed wide variation in elemental contents of Cr, Mn, Fe, Zn, Se, P including toxic pollutants, Sb and Hg. An attempt has been made to calculate daily dietary intake (DDI) for some nutrient elements from Indian fish. Elemental data for Cr, Cu, Fe, Hg, Se, and Zn have been compared with those from other fish exporting countries.
Keywords:Fish analysis  toxic pollutants  nutrients  biological reference materials  bioenvironmental indicator  aquatic environment  prawn  daily dietary intake (DDI)  instrumental neutron activation analysis (INAA)  international comparison
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