Impact of conditions of cultivation and adsorption on antimicrobial activity of marine bacteria |
| |
Authors: | E P Ivanova D V Nicolau N Yumoto T Taguchi K Okamoto Y Tatsu S Yoshikawa E P Ivanova |
| |
Institution: | (1) Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, pr. 100 let Vlkadivostoku 159, 690022 Vladivostok, Russia, RU;(2) Osaka National Research Institute, AIST, 1-8-31 Midorigaoka, Ikeda, Osaka 563, Japan, JP |
| |
Abstract: | The potency of free-living and animal-associated marine bacteria to produce antimicrobial substances has been studied in
491 strains isolated from northern and southern parts of the Pacific Ocean. A total of 26% (126 out of 491) of the strains
examined produced antimicrobial compounds against 11 test bacterial strains (TBS) including the fish pathogens Aeromonas hydrophila and Vibrio anquillarum. Antimicrobial substances (AS) produced by marine bacteria were especially active against Staphylococcus epidermidis, Proteus vulgaris, Enterococcus faecalis, and Candida albicans. Twelve strains, isolated from different sources, were chosen as promising candidates, producing a number of AS. Production
of AS varied within 24 to 72 h, increasing in a culture medium based on natural sea water with Br-ions, and after attachment
to polymeric surfaces. In order to study the influence of adsorption, selected strains with a high potential for antimicrobial
production were cultivated on polymeric surfaces with different hydrophobicities and chemical functionalities. These parameters
of the surface hydrophobicity (measured by means of water contact angles) and chemical functionality of the surfaces were
manipulated using the photo- and thermochemistry of a polymeric system (diazo-naphto-quinone/novolak) commonly used as a photoresistant
material in semiconducto-manufacturing. The highest antimicrobial activities occurred on hydrophilic surfaces (standard exposed
photoresistant films), whereas the number of attached cells was greater on hydrophobic surfaces, characterized as unexposed
resistant films. These results suggest that the chemical nature of induced hydrophilicity may also be a major factor in controlling
antimicrobial activity of adsorbed bacteria.
Received: 5 March 1997 / Accepted: 24 August 1997 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|