A multi-locus genetic assignment technique to assess sources of <Emphasis Type="Italic">Agaricia agaricites</Emphasis> larvae on coral reefs |
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Authors: | Email author" target="_blank">Daniel?A?BrazeauEmail author Paul?W?Sammarco Daniel?F?Gleason |
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Institution: | (1) Department of Pharmaceutical Sciences, University at Buffalo, Buffalo, NY 14260-1200, USA;(2) Louisiana Universities Marine Consortium (LUMCON), 8124 Hwy. 56, Chauvin, LA 70344, USA;(3) Department of Biology, Georgia Southern University, Statesboro, GA 30460-8042, USA |
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Abstract: | Assignment of individuals to populations based upon genetic data is an important ecological problem that requires many polymorphic
markers, often more than are available using single locus techniques. To demonstrate the utility of amplified fragment length
polymorphisms (AFLP) in studying larval dispersal and recruitment in coral populations, two sets of AFLP primers were used
to genotype colonies of the coral Agaricia agaricites Linnaeus from three widely separated geographic locations: the Bahamas (23°28′N, 75°42′W) and Key Largo, Florida (24°55′N,
80°31′W—two sites separated by 12 km) in 1995, and the Flower Garden Banks (FGB) in the Gulf of Mexico (27°55′N,93°36′W) in
1997. In addition to adult samples from each site, recruits were collected from settling plates placed on the East FGB for
1 year (1997–1998). The AFLP technique yielded 45 polymorphic markers. An analysis of molecular variance (AMOVA) showed significant
genetic differences among the four adult populations, even between the two Key Largo sites. The recruits were significantly
different from all adult populations except those from the FGB. Discriminant function analysis and the program AFLPOP were
used to assign individuals to populations. Using the adult AFLP-banding patterns to build the statistical models, both procedures
correctly assigned the majority of adults to their respective populations in simulations and assigned all but one of the recruits
to the Flower Garden population from where they were collected . The AFLP technique provides a simple and adaptable population
assignment method for studying recruitment processes in A. agaricites and other coral species.
Electronic Supplementary Material Supplementary material is available for this article at |
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