Localization of RubisCO and sulfur in endosymbiotic bacteria of the gutless marine oligochaete Inanidrilus leukodermatus (Annelida) |
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| Authors: | J Krieger O Giere N Dubilier |
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| Institution: | Zoological Institute and Zoological Museum, University of Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany Tel.: +49-40-428384234; Fax: +49-40-428383937 e-mail: jens.krieger@zoologie.uni-hamburg.de, DE
Max-Planck-Institute for Marine Microbiology, Celsiusstra?e 1, 28359 Bremen, Germany, DE
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| Abstract: | The chemoautotrophic potential of the two co-occurring larger and smaller bacterial endosymbionts of the gutless marine oligochaete
Inanidrilus leukodermatus was determined using immunocytochemistry. An antibody directed against the Form I of ribulose-1,5-bisphosphate carboxylase/oxygenase
(RubisCO), the key CO2-fixing enzyme of the Calvin–Benson cycle, consistently labeled the larger symbionts. Electron microscopic spectroscopy showed
that the larger symbionts contained sulfur in intracellular globules and to a lesser degree in the cytoplasm. The presence
of RubisCO and sulfur indicates that the larger endosymbionts of I. leukodermatus are chemoautotrophic sulfur-oxidizing bacteria. In contrast, no RubisCO or sulfur was detected in the smaller endosymbionts
of this host.
Received: 28 September 1999 / Accepted: 29 May 2000 |
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