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Nitrogen uptake characteristics of Chordaria flagelliformis (Phaeophyta) in batch mode and continuous mode experiments
Authors:T A Probyn  A R O Chapman
Institution:(1) Department of Biology, Dalhousie University, B3H 4J1 Halifax, Nova Scotia, Canada
Abstract:The uptake of NH 4 + and urea by Chordaria flagelliformis was measured in a perturbed system (batch mode) and in an apparent steady-state system (continuous mode). The maximum uptake rates (Vmax) measured in short-term depletion experiments greatly exceeded those determined under a steady nutrient supply. C. flagelliformis briefly exposed to high nutrient concentrations has an impressive capacity for rapid uptake (high Vmax)_which is uncoupled from growth. In contrast, under continous, homogenous low N concentrations the alga appears well equipped (low half-saturation constant, Km) to take up the available N. The results show that previous work based on batch mode uptake experiments probably overestimated the N requirements for the growth of many seaweeds.
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