Seasonal variations of ATPase activity and antioxidant defenses in gills of the mud crab <Emphasis Type="Italic">Scylla serrata</Emphasis> (Crustacea,Decapoda) |
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Authors: | Xianghui Kong Guizhong Wang Shaojing Li |
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Institution: | (1) Department of Oceanography, State Key Laboratory of Marine Environmental Science, Xiamen University, 361005 Xiamen, People’s Republic of China;(2) College of Life Science, Henna Normal University, 453007 Xinxiang, People’s Republic of China |
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Abstract: | The mud crab Scylla serrata is an important commercial crustacean being widely distributed along the southeastern coast of China. This crab and particularly
its gills are subjected to seasonal fluctuations of environmental factors due to direct exposure to seawater. The investigation
of seasonal variations of ATPase and antioxidant defenses in gills could be helpful for the understanding of physiological
regulation mechanism of seasonal adaptations. In this study, mud crabs were collected from the subtropical waters near Xiamen
island, Southeast China (24°26′46′′N, 118°04′04′′E), in August and November, 2002, and February and May, 2003, respectively,
being considered as specimens from summer, autumn, winter and spring, respectively. Only healthy intermoult male crabs without
carapace or appendage damage were used, having a carapace width of 7.05 ± 0.52 cm and a wet weight of 130 ± 20 g. The activities
of four ATPases and three antioxidation enzymes (superoxide dismutase, catalase, glutathione peroxidase) and the content of
malondialdehyde in gills were measured. The results showed that the activities of Na+, K+-ATPase, Mg2+-ATPase, Ca2+-ATPase and Ca2+, Mg2+-ATPase reached maximum levels similarly in summer and decreased in winter. The activities of superoxide dismutase and catalase
were higher in summer and lower in winter, with a significant decrease in winter compared to the other seasons (p < 0.01). Glutathione peroxidase activity was higher in summer, and lower in autumn, being significantly higher in summer or
winter than in spring or autumn (p < 0.01). Malondialdehyde content was higher in summer and lower in spring with significant differences among the different
seasons (p < 0.01). In summary, the obvious seasonal activity variations of four ATPases and antioxidant enzymes and the content of malondialdehyde
reflect a seasonal regulation of the physiological metabolising enzyme and the antioxidant capacity to cope with seasonal
alterations of environment factors such as fluctuating salinities and temperatures. |
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