假单胞菌AEBL3对土壤中呋喃丹的生物降解

从农药污染的农田土壤中分离到一株假单胞菌AEBL3，该菌能够以呋喃丹为唯一的碳源和氮源生长。使用AEBL3作为生物强化剂对模拟呋喃丹污染的土壤环境进行了生物修复试验。结果表明，接种AEBL3能够明显增加土壤中呋喃丹的降解率。降解菌AEBL3在土壤中具有一定的移动性，当从土壤表层加菌时，对0-7cm深土层中的呋喃丹都有很好的降解效果。在各种投加方式的比较中，以投加降解菌原液修复效果最好，缓冲液悬浮的菌细胞次之，砂粒混合物的效果最差。16S rRNA的变性梯度电泳(DGGE)结果揭示了生物修复过程中土壤微生物菌群结构中的动态变化。

Characterization of Superior Strain AEBL3 of Degrading Carbamate Pesticides

A strain, Pseudomonas sp. AEBL3, which was isolated from soils exposed to repeated spills of carbamate pesticides, was capable of utilizing carbofuran as a sole source of carbon and nitrogen. AEBL3 was used as inoculant to bioremediate carbofuran simulated-contaminating soil. The result shows degradation rate of carbofuran in the soil was increased remarkably by the treatment. AEBL3 is mobile in the soil to a certain extent. When AEBL3 was added to the soil surface, it had a strong ability to degrade the carbofuran in 0-7cm deep soil. The effects on bioremediation of different inoculation formulations were compared. The original liquid formulation was the best. The buffer solution was the second and mixture with sand was the worst. Denaturing gradient gel electrophoresis(DGGE) of 16S rRNA genes revealed a dynamic change in the microbial community structure of the soil during bioremediation.