| 低温贫营养好氧反硝化细菌SY13的反硝化特性 |
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| 引用本文： | 苏俊峰,杨少斐,黄廷林,魏巍,张海涵,郭琳.低温贫营养好氧反硝化细菌SY13的反硝化特性[J].城市环境与城市生态,2013(6):39-42. |
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| 作者姓名： | 苏俊峰 杨少斐 黄廷林 魏巍 张海涵 郭琳 |
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| 作者单位： | [1]西安建筑科技大学环境与市政工程学院,西安710055 [2]清华大学国家环境保护环境微生物利用与安全控制重点实验室,北京100084 |
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| 基金项目： | 基金项目：国家科技支撑计划（2012BAC04802）;国家环境保护环境微生物利用与安全控制重点实验室开放基金（MARc2012D001）;陕西省教育厅专项科研计划项目（12JK0896）;陕西省教育厅重点实验室科学研究计划项目（12JS051） |
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| 摘 要： | 从底泥中分离出1株低温贫营养好氧反硝化细菌SY13，经常规生理生化鉴定和16SrDNA测序，鉴定出细菌SY13属于Acinetobactersp．。考察了温度、pH、C／N比及接种量对菌株SY13硝酸盐还原活性的影响，初始硝酸盐浓度为15mg／L左右，温度为15℃时低温贫营养好氧反硝化细菌SY13的硝酸盐去除率为49．26％，在中性环境适应性较强，pH值为7．0时72h的硝氮去除率达到58．08％，随着C／N比不断增加，菌株SY13硝酸盐的去除效果逐渐增强，接种量为10％时，菌株SY13培养72h后的硝氮去除率可达到59．62％。
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| 关 键 词： | 细菌SY13（Acinetobacter sp ） 贫营养细菌 低温好氧反硝化 生物脱氮 |
Aerobic Denitrification of Bacteria SY13 （Acinetobacter sp.） under Low Temperature and Poor Nutrition |
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| SU Jun-feng,YANG Shao-fei,HUANG Ting-lin,WEI Wei,ZHANG Hai-han,GUO Lin.Aerobic Denitrification of Bacteria SY13 （Acinetobacter sp.） under Low Temperature and Poor Nutrition[J].Urban Environment & Urban Ecology,2013(6):39-42. |
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| Authors: | SU Jun-feng YANG Shao-fei HUANG Ting-lin WEI Wei ZHANG Hai-han GUO Lin |
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| Institution: | 1. School of Environmental and Municipal Engineering, Xi＇an University of Architecture and Technology, Xi＇an 710055, China; 2. State Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Tsinghua University, Beijing 100084,China) |
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| Abstract: | Aerobic denitrifying bacteria SY13 was isolated from the sediment under low temperatures and poor nutrition conditions. According to its physiological-biochemical identification, 16SrDNA gene sequence analysis, and pbylogenetic tree analysis, the bacteria SY13 was identified to belong to A cinetobacter sp.. The effects of temperature, pH,C/N ratio and bacterial inoculation on the activity of nitrate reduction were investigated. The strain SY13 was cultured in the medium containing 15 mg/L nitrate. When the temperature was 15℃, the strain SY13 had a desirable ability of aerobic denitrification, and the nitrate nitrogen removal rate was 49.26%. When the pH was 7.0, the removal efficiency of nitrate nitrogen was 58.08% after 72 h. As the C/N ratio increased to〉3.0, along with the increase of carbon-nitrogen ratio, the denitrifying rate increased. When the Acinetobactersp. was inoculated at a dose of 15%, the nitrate removal efficiency of strain SY13 reached 59.62%. |
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| Keywords: | bacteria SY13 （Acinetobacter sp ） low temperatures and poor nutrition aerobic denitrification nitrogenremoval |
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