兔子宫内膜蛋白等电聚焦电泳柱状胶与板状胶电泳效果的比较

哺乳动物早期胚胎发育过程中，胚泡附植的成功与否对早期胚胎的发育十分重要．为了有效地弄清胚泡附植的分子机制，本研究从分析蛋白质的一种生化技术-等电聚焦电泳着手，利用兔子宫内膜蛋白作为实验材料，探讨了柱状胶与板状胶等电聚焦电泳分离蛋白质的电泳效果．研究结果表明：在相同情况下，柱状胶与板状胶的蛋白质分布有一些差别，pH梯度分布也不一致；板状胶比柱状胶pH值分布宽、蛋白质谱带多、分辨率高；板状胶酸性端只有1—2cm没有蛋白带、蛋白带分布比较均匀且压得比较紧．这说明在用子宫内膜作为实验材料进行电泳时用板状胶进行电泳的结果较好．

Comparision of Electrophoresis Effect of Tube gel and Board gel in IEF- PAGE

Implantation is crucial to the embryo development. In order to know the molecular mechanism of embryo implantation clearly, the electrophoresis effect of tube gel and board gel was investigated in the present study via a biochemical technique called isoelectric focusing - polyacrylamide gel electrophoresis (IEF - PAGE) using the rabbit endometrium as the experimental materials. The results demonstrated that there were some difference in the tube gel and board gel and there were different pH gradients in the two shapes; the board gel had the wider protein band diffusion and clearer differentiation than those of the tube gel; In addition, there were only 1 - 2cm left where there weren't any protein bands and proteins were dispersed regularly in the board gel. The above conclusions showed that board gel was better than tube gelin analyzing the proteins of rabbit endometrium, which was beneficial for improving the differentiation of the protein spots in two - dimensional electrophoresis.