大鼠胚胎期PFOS暴露致出生早期死亡及神经系统发育影响的基因组学分析

为了阐明PFOS导致新生儿死亡的可能原因和机制,探讨PFOS致死与神经系统发育的相互联系,利用基因芯片技术,观察了经PFOS饲料染毒后出生1和7d大鼠脑组织基因表达情况,通过基因组学(Gene Ontology)和生物路径(Pathway)对差异基因的功能和相互联系进行分析.结果显示,PFOS染毒后,出生1、7d的大鼠脑皮质组织分别有864、642条基因发生差异表达,差异基因涉及的与PFOS致死相关的生物过程包括中枢神经系统发育、血循环系统、刺激应答、骨骼发育、氧化应激、心脏功能以及pH值调节等.结果表明,PFOS导致的出生早期死亡与神经系统发育有关,PFOS可能通过改变脑组织血氧平衡,影响正常的中枢神经系统功能和发育过程而使新生儿的存活率下降.

Genomic Analysis of Effects of Gestational PFOS Exposure on Neonatal Death and the Nervous System Development in Rat

To investigate the potential mechanism of PFOS-induced neonatal mortality at the molecular level and to find out the interaction between PFOS-induced mortality and developmental neurotoxicity,the RatRef-12 Expression BeadChip was used to identify changes in gene expression at postnatal days(PNDs)1 and 7.The Gene Ontology for translation genes into relative functions and the pathway for graphical representations of gene interactions were both employed for further analysis of the expression data.There are 864 and 642 genes significantly affected by PFOS at PNDs1 and 7,respectively(p <0.05).Some of bioprocesses related to differentially expressed genes have been involved in the PFOS-induced mortality including:death,the central nervous system development,blood circulation,response to stimulus,bone development,response to hypoxia,heart process and regulation of pH.Results of this study indicate that the disruption of nervous system may lead to PFOS-induced neonatal mortality through the disruption of the brain blood and oxygen system and interference with the normal functional process of brain.