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纳米与微米尺度氧化锌体外遗传毒作用特征的比较研究
引用本文:梁春柳,朱江波,朱玉平,姚朗,张天宝.纳米与微米尺度氧化锌体外遗传毒作用特征的比较研究[J].生态毒理学报,2012,7(3):299-304.
作者姓名:梁春柳  朱江波  朱玉平  姚朗  张天宝
作者单位:1. 南方医科大学公共卫生与热带医学学院毒理学系,广州,510515
2. 第二军医大学卫生毒理学教研室,上海,200433
基金项目:国家重大科学计划子课题(2006CB932505);上海市科委纳米专项基金(0752nm020)
摘    要:为揭示纳米氧化锌(ZnO)遗传毒作用特征,以便为纳米材料的安全性评价提供科学依据,利用胞质分裂阻滞微核细胞组学实验和碱性彗星实验比较了纳米和微米尺度ZnO的遗传毒性.结果显示:①微米尺度ZnO在0.02~2.5 μg·mL-1剂量下对微核、核质桥、核芽和核分裂指数(nucleus divided index,NDI)均...

关 键 词:纳米氧化锌  微米氧化锌  彗星实验  微核  核质桥  核芽
收稿时间:2011/8/29 0:00:00
修稿时间:2011/10/24 0:00:00

Comparative Study on in Vitro Genotoxicity Caused by Nano- and Micro-Scale ZnO
Liang Chunliu,Zhu Jiangbo,Zhu Yuping,Yao Lang and Zhang Tianbao.Comparative Study on in Vitro Genotoxicity Caused by Nano- and Micro-Scale ZnO[J].Asian Journal of Ecotoxicology,2012,7(3):299-304.
Authors:Liang Chunliu  Zhu Jiangbo  Zhu Yuping  Yao Lang and Zhang Tianbao
Institution:Department of Toxicology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China;Department of Health Toxicology, the Second Military Medical University, Shanghai 200433, China;Department of Health Toxicology, the Second Military Medical University, Shanghai 200433, China;Department of Toxicology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China;Department of Health Toxicology, the Second Military Medical University, Shanghai 200433, China
Abstract:To investigate the characteristics of genotoxicity of nano-ZnO and so as to provide a scientific basis for safety assessment of nanomaterials,the comparison of genotoxicity between nano-and micro-scale ZnO was conducted using cytokinesis-block micronucleus cytome test and alkaline comet assays in vitro.Results were as follows:① Micro-ZnO could not significantly influence the indexes such as micronucleus,nucleoplasmic bridge,nuclear bud and nuclear divided index(NDI) at the dosage of 0.02-2.5 μg·mL-1(P>0.05).However,nano-ZnO at the dosage higher than 0.5 μg·mL-1 led to an increase in the numbers of total micronucleus,type Ⅰ micronucleus and nuclear bud in a dose-dependent manner(P<0.05).Compared with the control group,significant differences were observed in the numbers of type Ⅱ micronucleus,nucleoplasmic bridge and NDI in nano-ZnO group with the dosage of 2.5 μg·mL-1(P<0.05).② No significant differences were found in all the tested indexes after exposure to 2.5 μg·mL-1 of micro-ZnO for 9,18 and 24 h,compared with the control group(P>0.05).However,the numbers of total micronucleus,type Ⅰ micronucleus and nucleoplasmic bridge exhibited a time-effect relationship in nano-ZnO group.③ Nano-ZnO caused DNA damage at 0.5 μg·mL-1,but DNA damage was not observed in micro-ZnO group until the dosage of 2.5 μg·mL-1.The study demonstrated that the properties and strength of genotoxicity were significantly different between nano-ZnO and micro-ZnO.Nano-ZnO could induce the generation of type Ⅰ micronucleus,type Ⅱ micronucleus,nuclear bud and nucleoplasmic bridge and cause DNA damage,whereas only DNA damage was caused by micro-ZnO at relatively high doses.
Keywords:nano-ZnO  micro-ZnO  comet assay  micronucleus  nucleoplasmic bridge  nuclear bud
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