条斑紫菜中磷和钙的亚细胞分布及其与富集砷的关系

研究条斑紫菜中磷和钙的亚细胞分布,并探讨其与条斑紫菜富集砷的关系。结果表明:条斑紫菜吸收磷、钙主要分布在细胞壁组分中,平均占总磷含量的53.9%,占总钙含量的61.25%,其次为细胞液。与对照组相比(砷暴露浓度0.5 mg·L~(-1)),在添加磷浓度为0.1 mg·L~(-1)和1.0 mg·L~(-1)处理组中,条斑紫菜中磷、砷之间呈现协同效应;当添加磷浓度为5.0 mg·L~(-1)和10.0 mg·L~(-1)时,条斑紫菜中磷、砷之间则呈现拮抗作用,砷的富集量分别比照组下降61.37%和72.73%。经添加钙液暴露过的条斑紫菜,当钙暴露浓度为1 000 mg·L~(-1)时其对砷的富集量比对照组减少15.51%。可知,5.0 mg·L~(-1)磷处理条斑紫菜,条斑紫菜对砷的富集受到明显的抑制。

The Subcellular Fate of Phosphorus and Calcium in the Seaweed Porphyra yezoensis and Its Relationship with the Arsenic Accumulation

The subcellular distribution of phosphorus (P) and calcium (Ca) in P. yezoensis was studied and the relationship with its high accumulation of arsenic was also discussed. The results showed that P and Ca were mainly accumulated in the cell wall, accounting for about 53.9% of total P and about 61.25% of total Ca. The second largest accumulation of P and Ca was in the cytoplasm. Compared with the control group (only treated with 0.5 mg?L^-1 As), the synergistic effect occurred in the uptake of P and As for groups also exposed to 0.1 and 1.0 mg?L^-1 P, while the antagonistic effect occurred for 5.0 and 10.0 mg?L^-1 P pretreatment groups, and the accumulated As decreased by 61.37% and 72.37% respectively. For the group exposed to Ca (1 000 mg?L^-1), the accumulated As concentration decreased by 15.51% compared to the control group. It could be concluded that 5.0 mg?L^-1 P could significantly inhibit the accumulation of As in P. yezoensis.