全氟十二烷酸(PFDoA)慢性暴露干扰大鼠肝脏磷酸化蛋白质水平

全氟烷基类化合物(PFASs)是一类新型持久性有机污染物,因其独特的理化性质被广泛应用于工业和商业领域,对生物体具有一定的毒性作用。为探究全氟十二烷酸(PFDo A)致肝脏毒性作用机制,选择雄性大鼠为受试生物,采用2-DE蛋白质组学技术与Pro Q Diamond dye磷酸化蛋白染色结合的方法初步研究了不同剂量PFDo A暴露110 d后大鼠肝脏蛋白磷酸化水平的变化。结果表明,30个磷酸化蛋白表达水平在PFDo A处理后发生显著变化,其中,经过MALDI-TOF/TOF质谱分析,成功鉴定18个蛋白点。经过生物信息学分析,发现这些蛋白主要涉及糖脂代谢、氨基酸代谢、应激防御及电子传递等途径。以上结果有助于进一步从翻译后修饰水平了解PFDo A的肝脏毒性作用。

Chronic Exposure of PFDoA Affect the Phosphorylation Levels of Proteins in Rat Liver

Perfluoroalkyl and polyfluoroalkyl substances (PFASs), an emerging kind of persistent organic pollutant, have been widely used in commercial and industrial products due to their unique physicochemical properties. PFASs have various toxicities to the living system. Perfluorododecanoic acid (PFDoA) with twelve carbon atoms, has broad industrial applications and is widely distributed in both wildlife and the environment. However, unlike other PFASs with short carbon chains, up to date limited studies have been performed on the toxic effects of PFDoA on animals. To reveal the mechanism of PFDoA hepatotoxicity, male rats were exposed to PFDoA for 110 days and the phosphoprotein profiles in the rat liver were analyzed by 2-DE methods coupled with Pro-Q Dimond phosphoprotein stain (Pro-Q DPS). The results showed significant change in the phosphorylation levels of 30 proteins after PFDoA exposure. Among them, 18 proteins were identified successfully by MALDI-TOF/TOF MS analysis. After bioinformatics analysis, these identified phosphoproteins were found to be involved in lipid metabolism, glucose metabolism, stress and immune response, electron transport, and so on. The results presented in this study will provide basic data for further study of the mechanism of PFDoA hepatotoxicity at the post-translational modification level.