| 镉诱导大鼠肾细胞凋亡及其机理的研究 |
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| 引用本文： | 贾广乐,王众,吴绍强,林祥梅,韩雪清,刘建,梅琳,张旻.镉诱导大鼠肾细胞凋亡及其机理的研究[J].生态毒理学报,2008,3(3):268-273. |
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| 作者姓名： | 贾广乐 王众 吴绍强 林祥梅 韩雪清 刘建 梅琳 张旻 |
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| 作者单位： | 中国检验检疫科学研究院,北京,100029 |
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| 基金项目： | 国家科研院所基本科研业务费资助项目(No. 2007JK022) |
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| 摘 要： | 为探讨镉(Cd)致大鼠肾细胞(NRK cell)凋亡的死亡受体途径,分别将终浓度为0、10、20、40、60μmol·L-1的CdCl2添加到培养液中,对NRK细胞暴露6h.应用流式细胞仪检测NRK细胞凋亡率;分光光度计检测Caspase-8蛋白活性;半定量RT-PCR检测Caspase-8、Fas的mRNA表达.结果表明,随着CdCl2暴露浓度的升高,NRK细胞凋亡率升高,Caspase-8蛋白活性增强,Caspase-8、Fas的mRNA表达增强,并呈现剂量-效应关系.说明镉可以通过死亡受体这条通路来引起NRK细胞凋亡.
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| 关 键 词： | 镉 NRK细胞 死亡受体途径 |
| 收稿时间： | 2/2/2008 12:00:00 AM |
| 修稿时间： | 2008/3/23 0:00:00 |
Study on the Mechanisms of NRK Cells Apoptosis Induced by Cadmium |
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| JIA Guang-le,WANG Zhong,WU Shao-qiang,LIN Xiang-mei,HAN Xue-qing,LIU Jian,MEI Lin,ZHANG Min.Study on the Mechanisms of NRK Cells Apoptosis Induced by Cadmium[J].Asian Journal of Ecotoxicology,2008,3(3):268-273. |
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| Authors: | JIA Guang-le WANG Zhong WU Shao-qiang LIN Xiang-mei HAN Xue-qing LIU Jian MEI Lin ZHANG Min |
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| Abstract: | In order to study the death-receptor pathway in NRK cell apoptosis induced by cadmium, different concentrations of cadmium chloride (0, 10, 20, 40, 60mmol·L-1) were added to NRK cell culture medium and cultured for 6h. Apoptosis rate, Caspase-8 activity and expression level of mRNA of Caspase-8 and Fas were detected by flow cytometry, spectrophotometer and RT-PCR, respectively. Results showed that concomitant dose-dependent increases in apoptosis rate, Caspase-8 activity and the expression level of Caspase-8 and Fas mRNA were found, which indicated apoptosis of NRK cells could be induced by cadmium through death-receptor pathway. |
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| Keywords: | cadmium NRK cell death-receptor pathway |
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