低浓度阿特拉津长期暴露对鲫鱼DNA的影响

运用随机扩增多态性DNA(RAPD)技术在分子生物学水平上评价了低浓度阿特拉津长期暴露对鲫鱼DNA的影响.结果表明:0.006mg·L-1以上浓度(试验浓度系列为0.006、0.023、0.094、0.375、1.5、3mg·L-1)的阿特拉津持续暴露60d均会对鲫鱼的DNA产生影响;经筛选,确定有34条引物能够扩增出鲫鱼基因组DNA,其中有8条引物能检测出阿特拉津对鲫鱼基因组DNA影响的差异:各浓度组鲫鱼DNA的RAPD扩增产物条带均出现不同程度缺失;在较低浓度长期暴露下阿特拉津对鲫鱼基因组DNA的损伤无明显的剂量-效应关系,阿特拉津毒性在0.375mg·L-1时突然增强,但这一趋势并未向更高浓度组延续(1.5mg·L-1组表现出的毒性弱于0.375mg·L-1组和3mg·L-1组).

Effects of Long Term Exposure to Low Concentration Atrazine on the DNA of Carassius auratus

This article intends to study the effects of continuous exposure to low-concentration atrazine on the DNA of crucian(Carassius auratus). In this experiment, a random amplified polymorphic DNA(RAPD) technique is adopted to analyze the influences of continuous exposure(60d)to 0.006, 0.023, 0.094, 0.375, 1.5 and 3mg·L-1 atrazine on the DNA of crucian. Results show that 8 primers among 34 primers used can produce different amplified fragments in crucian genomic DNA after 60d exposure. On the condition that the concentration of atrazine remains above 0.006mg·L-1, it can influence crucian's DNA. Loss of different degrees of the amplified results is found in all treatments. There are no obvious dose-effect relationships between atrazine concentrations and DNA damages on condition of low concentration and long-term exposure, for the toxicity of 1.5mg·L-1 atrazine is less than that of 0.375mg·L-1 and 3mg·L-1.