纳米水滑石与Hela细胞的生物相容性研究

为了探讨纳米水滑石与Hela细胞的生物相容性,采用单细胞凝胶电泳法检测了纳米水滑石对Hela细胞DNA的损伤,并采用H2DCF-DA荧光法检测了纳米水滑石对Hela细胞活性氧簇的影响.结果发现,随着纳米水滑石浓度的升高(0~800μg·mL-1),Hela细胞尾部DNA含量、尾距及胞内活性氧簇含量均呈逐渐升高趋势;较低浓度(50、100μg·mL-1)纳米水滑石处理后,Hela细胞尾部DNA含量、尾距及胞内活性氧簇含量与对照组无显著性差异(p>0.05),而较高浓度(200、400、800μg·mL-1)纳米水滑石处理后,Hela细胞尾部DNA含量、尾距及胞内活性氧簇含量与对照组差异显著(p<0.05,p<0.01).以上结果表明,较高浓度(≥200μg·mL-1)的纳米水滑石可对Hela细胞DNA产生损伤,而较低浓度(≤100μg·mL-1)的纳米水滑石则具有较好的生物相容性,有可能作为载体应用于医药领域.

Study on Biocompatibility of Nano-Layered Double Hydroxides with Hela Cells

In order to explore the biocompatibility of nano-layered double hydroxides (nano-LDHs) with Hela cells, a single cell gel electrophoresis and H2DCF-DA fluorescent coloration assay were used to analyze the DNA damage and the change of reactive oxygen species (ROS) in Hela cells exposed to nano-LDHs. Results showed that, with the increase of concentrations of nano-LDHs (0~800μg·mL-1), the Tail DNA%, Tail Moment and ROS contents in Hela cells increased gradually; There were significant differences (p<0.05, p<0.01) in Tail DNA%, Tail Moment and ROS contents between the high concentration groups (200、400、 800μg·mL-1) and the control group, while no significant differences (p>0.05) were found between the low concentration groups (50, 100μg·mL-1) and the control group. The above results suggested that the high concentration nano-LDHs (≥200μg·mL-1) could induce the DNA damage in Hela cells and the low concentration nano-LDHs (≤100μg·mL-1) had a good biocompatibility with Hela cells.