利用模式动物秀丽线虫建立环境毒物毒性的评估研究体系

模式动物秀丽线虫(Caenorhabditis elegans)在环境科学与生态毒理学研究中发挥着越来越重要的作用,目前利用秀丽线虫已初步建立起较为完善的环境毒物毒性评估体系.该评估体系具体由如下一些指标构成:致死率、最长寿命与半数致死天数、细胞凋亡、个体发育、生殖、运动行为、乙酰胆碱酯酶活性、学习行为、记忆行为、转基因品系、突变体、基因表达模式.论文系统总结了近十多年来利用秀丽线虫进行环境毒物毒性评估的研究体系,并对各个指标的研究方法、研究现状、存在的问题及应用中需要注意的地方进行了讨论。

Establishment of Toxicity Evaluation System Using Model Organism of Caenorhabditis elegans

Model organism of Caenorhabditis elegans has received more and more attention for its important contribution to the study in environmental science and ecotoxicology. So far, relatively perfect toxicity evaluation system has been established using C. elegans. This toxicity evaluation system contains the indicators of lethality, maximum and mean lifespan, apoptosis, development, reproduction, locomotion behavior, acetylcholinesterase activity, learning, memory, transgenic strain, mutant, and expression pattern of gene. The toxicity evaluation system using C. elegans in recent decades was systematically reviewed, and the methods, research progresses, advantages and disadvantages for each indicator were discussed in this paper.