| 应用体内和体外方法评价原油水溶性组分和原油污染的地表水对淡水生物的内分泌干扰潜力 |
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| 作者姓名： | - |
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| 摘 要： | 目前关于原油对淡水水生脊椎动物内分泌信号的潜在影响的认知有限。本文以非洲爪蟾(爪蟾属，X. laevis)蝌蚪和莫桑比克罗非鱼幼鱼(口孵非鲫属，O. mossambicus)为研究对象，选择风化原油和未风化提炼后的原油这2种原油的水溶性组分(WAFs)进行暴露实验，选择出可作为内分泌信号改变的生物标志物的基因，对其表达进行了研究。此外，将X. laevis蝌蚪暴露于靠近地下石油库的地表水中，对上述基因表达做了定量分析。利用重组酵母对原油、原油水溶性组分和地表水的(抗)雌激素和(抗)雄激素性进行了评价。在X. laevis对2种原油WAFs的响应中，甲状腺激素受体β表达均显著下调，而另一种与甲状腺相关的基因，2型脱碘酶，在O. mossambicus暴露于高浓度的原油WAF时被上调。此外，这2种WAFs都改变了与脂肪生成相关的过氧化物酶体增殖物激活受体γ在X. laevis中的表达。原油和WAFs在体外表现出抗雌激素和抗雄性激素的活性。然而，O. mossambicus雄激素受体2作为唯一一个代表生殖系统的基因，受到WAF暴露的影响显著。在地表水样品中发现了雌激素、抗雌激素和抗雄激素的作用；但是，在暴露于地表水的X. laevis中没有观察到任何基因表达的明显变化。在所用的2种模式生物以及2种原油中，响应各不相同。尽管如此，这些数据提供了证据证明原油污染可能会因内分泌信号转变对淡水鱼和两栖动物的健康造成不利影响。
精选自Truter, J. C., van Wyk, J. H., Oberholster, P. J., Botha, A.-M. and Mokwena, L. M. (2017), An evaluation of the endocrine disruptive potential of crude oil water accommodated fractions and crude oil contaminated surface water to freshwater organisms using in vitroand in vivo approaches. Environmental Toxicology and Chemistry, 36: 1330–1342. doi: 10.1002/etc.3665
详情请见http://onlinelibrary.wiley.com/doi/10.1002/etc.3665/full
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An Evaluation of the Endocrine Disruptive Potential of Crude Oil Water Accommodated Fractions and Crude Oil Contaminated Surface Water to Freshwater Organisms Using in vitro and in vivo Approaches |
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| -.An Evaluation of the Endocrine Disruptive Potential of Crude Oil Water Accommodated Fractions and Crude Oil Contaminated Surface Water to Freshwater Organisms Using in vitro and in vivo Approaches[J].Asian Journal of Ecotoxicology,2017,12(3). |
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| Authors: | J Christoff Truter Johannes H van Wyk Paul J Oberholster Anna-Maria Both Lucky M Mokwena |
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| Institution: | 1. Department of Botany and Zoology, Stellenbosch University, Stellenbosch, South Africa
2. CSIR Natural Resources and the Environment, Stellenbosch, South Africa
3. Department of Genetics, Stellenbosch University, Stellenbosch, South Africa
4. Central Analytical Facility, Mass Spectrometry Unit, University of Stellenbosch, Stellenbosch, South Africa |
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| Abstract: | |
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| Keywords: | Endocrine disrupting chemical Freshwater toxicology Polycyclic aromatic hydrocarbon Oil spill Amphibian |
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