三氯卡班可以影响肾小管上皮细胞的屏障功能

三氯卡班(TCC)是一种被广泛应用于个人护理用品中的广谱型亲脂性杀菌剂,已在多种环境介质和生物体中检出。因其潜在的环境蓄积、生物累积和生物毒性效应,日益受到学者们的关注。借助TCC对NRK-52E(大鼠肾小管上皮细胞)的毒性暴露实验,通过检测细胞活力、以及与跨膜电阻和紧密连接相关的连接黏附分子1(JAM~(-1),junctional adhesion molecule 1)的蛋白表达水平,研究了TCC潜在的肾脏毒性效应。结果显示,10μmol·L~(-1)TCC处理48 h时培养细胞呈现不规则的集落;10μmol·L~(-1)和20μmol·L~(-1)TCC处理NRK-52E 24 h、48 h和72 h后可以显著抑制细胞生长;3.57μmol·L~(-1)TCC(生长抑制的48 hIC20)处理NRK-52E 48 h可以显著抑制细胞间紧密连接蛋白JAM~(-1)的表达量,并降低跨膜电阻,影响肾脏的屏障功能。本研究的结果能够为进一步揭示TCC对动物的毒害机制、评估其对动物的健康风险提供数据支持。

Triclocarban Alters Barrier Function in NRK-52E Cells

Triclocarban (TCC) is a broad-spectrum,lipophilic,antimicrobial additive that is largely applied in a variety of personal care products (PCPs).Now,this chemical has been detected in various environmental matrices including sediments,fishes and water.Because of its potential accumulation capacity and biological toxicity,TCC is now increasingly drawing the attention of the researchers.In this study,the potential renal toxicity was evaluated using NRK-52E cells,and we analyzed cell viability,transepithelial electrical resistance (TER) and the protein levels of junctional adhesion molecule 1 (JAM-l) which is closely related to cellular tight junction.The results showed that irregular colonies occurred after exposing NRK-52E cells to 10 μmol·L-1 TCC for 48 h,and the cell growth was also significantly inhibited in a dose-and time-dependent manners.Also,the TER and the protein levels of JAM-1 decreased after treatment with 3.57 μmol·L~ TCC for 48 h,implying the potential adverse effect of TCC on the barrier function of the kidney.Therefore,this study illustrated the in vitro nephrotoxicity of TCC,and the results can provide evidences for future study on toxicity mechanism of TCC and the possible health assessment on animals.