污水处理厂空气介质抗生素抗性基因的分布

考察了污水处理厂空气介质中典型的抗性基因(antibiotic resistance genes,ARGs)污染水平和浓度分布,并通过16S r RNA高通量技术对样品进行亲缘性及溯源研究。结果表明,在污水厂空气样品中8种抗生素抗性基因的检出率均超过50%,其中tet C、sul1、sul2和erm B检出率为100%。在曝气池和污泥脱水车间空气样品中8种抗性基因检出率均为100%。对其中的sul1、sul2、tet G和tet X共4种ARGs的定量分析结果表明,以上4种基因的相对浓度范围在102~105copies·ng~(-1)DNA之间,与邻近居民区空气样品抗性基因浓度处于同一水平;空气样品16S r RNA高通量测序聚类分析结果显示,居民区空气与污水厂园区内空气有较高的种群相似度,污水厂处理单元对其邻近区域的空气介质微生物组成影响较大。

Distribution of Airborne Antibiotic Resistance Gene in Wastewater Treatment Plant

The pollution level and concentration distribution of the typical antibiotic resistance genes (ARGs) in the air of WWTP were investigated, then their affinity and emission sources were traced using 16S rRNA based high-throughput sequencing in the present study. Results showed that the positive detection rates were higher than 50% for the eight target ARGs in the air samples of WWTP and four of them (tetC, sul1, sul2 and ermB) were 100%. Moreover, all the ARGs in the air samples were 100% detected near the aeration tank and sludge dewatering tank. The quantitative analysis of four ARGs (sul1, sul2, tetG and tetX) showed that their relative concentration range is 10²~10⁵ copies·ng^-1 DNA, which was comparable with that of the air samples in the residential area nearby. Cluster analysis based on 16S rRNA high-throughput sequencing indicated that microbial community in the air in residential area had a high similarity with that in the WWTP, and treatment process in WWTP had a significant influence on the microbial composition of its ambient environment.