纳米二氧化硅对秀丽线虫的毒性作用研究

为探讨二氧化硅纳米颗粒(SiNP)的毒性作用.以微米SiO2和空白做对照,将尺寸为20和60 nm的SiNP分别以0.2,1.0和5.0 g·L-13个浓度对模式生物秀丽隐杆线虫进行染毒.通过第一代和第二代线虫体长、身体弯曲频率、头部摆动频率以及第一代线虫后代数目、世代时间等发育、运动和生殖的相关评价指标,对SiNP毒...

Toxicity of SiO2 Nanoparticles to Caenorhabditis Elegans

The aim of this study was to investigate the toxicity of SiO₂ nanoparticles by using a model organism C. elegans. C. elegans were treated with 0.2, 1.0 and 5.0 g·L^-1 of 20 nm or 60 nm SiO₂ nanoparticles, and the control group were treated with 5.0 g·L^-1 of SiO₂ microparticle or 0.9% saline. The toxicity was detected using a battery of bio-tests. The indicator included body size, body bends, head thrashes of first- and second-generation C. elegans, brood type and generation time of first-generation C. elegans. Compared with control and microparticle groups, abnormities in development and locomotion were induced in the first-generation and second-generation C. elegans which were exposed to 5.0 g·L^-1 20 and 60 nm SiO₂ nanoparticles (p<0.01). Abnormities of reproductive indicators were observed in brood type and generation time when exposure dose of 60 nm SiO2 nanoparticles exceeded 0.2 g·L^-1 (p<0.01). 20 nm SiO₂ nanoparticles had no significant toxicity on the reproduction of C. elegans. Statistical analysis showed a significant difference between the toxicities of SiO₂ nano- and micronparticle treatments at 5 g·L^-1 SiO₂ nanoparticle solution. The results showed that the scale and concentrations of SiO₂ can affect its toxicity on development, locomotion behavior and reproduction of C. elegans. The above results could provide a reference for determining the exposure limit standard for SiO₂ nanoparticles.