首页 | 本学科首页   官方微博 | 高级检索  
     检索      

空气负离子暴露对秀丽隐杆线虫(Caenorhabditis elegans)的生物学效应
引用本文:蔡克周,刘雪兰,郭巧芝,许永建,陈华,余增亮.空气负离子暴露对秀丽隐杆线虫(Caenorhabditis elegans)的生物学效应[J].生态毒理学报,2007,2(4):396-401.
作者姓名:蔡克周  刘雪兰  郭巧芝  许永建  陈华  余增亮
作者单位:1. 中国科学院等离子体物理研究所,离子束生物工程重点实验室,合肥,230031
2. 安徽农业大学动物科技学院,合肥,230036
3. 中粮集团丰原生物技术研究中心,蚌埠,233010
基金项目:中国科学院知识创新工程项目
摘    要:利用模式生物秀丽隐杆线虫(Caenorhabditis elegans)研究了电晕法空气负离子(Electrically generated negative air ions,ENIs)暴露的生物学效应.将成年野生型和mev-1突变型线虫及新鲜虫卵持续暴露于空气负离子中(空气负离子剂量1×105~1.5×105ions·cm-3),观察空气负离子暴露对线虫发育周期、寿命、繁殖性能和性腺细胞凋亡的影响.结果表明:空气负离子暴露可显著加速野生型和mev-1突变型线虫发育(p<0.05),缩短寿命(p<0.05),与野生型相比,空气负离子暴露对mev-1突变线虫寿命影响更大(p<0.05);空气负离子暴露可显著降低线虫后代数量(p<0.05),原因可能在于空气负离子暴露显著诱导性腺细胞凋亡的增加(p<0.05).以上结果表明,持续空气负离子暴露对线虫具有损伤作用,mev-1基因突变导致线虫对空气负离子暴露更加敏感.

关 键 词:空气负离子  秀丽隐杆线虫  发育  寿命  繁殖性能
文章编号:1673-5897(2007)4-396-06
收稿时间:2007-10-27
修稿时间:2007-11-08

Effects of Exposure to Electrically Generated Negative Air Ions (ENIs)on the Nematode Caenorhabditis Elegans
CAI Ke-zhou,LIU Xue-lan,GUO Qiao-zhi,XU Yong-jian,CHEN Hua and YU Zeng-liang.Effects of Exposure to Electrically Generated Negative Air Ions (ENIs)on the Nematode Caenorhabditis Elegans[J].Asian Journal of Ecotoxicology,2007,2(4):396-401.
Authors:CAI Ke-zhou  LIU Xue-lan  GUO Qiao-zhi  XU Yong-jian  CHEN Hua and YU Zeng-liang
Abstract:The model organism Caenorhabditis elegans was used to study the biological effects induced by electrically generated negative air ions(ENIs)exposure. Young adult wild type and mev-1 mutant hermaphrodites were exposed to ENIs(the density of ENIs was 1×105~1.5×105ions·cm-3), and the development period, the lifespan, the broodsize and the germline cell apoptotisis of C. elegans were detected. Results showed that the development periods of two C. elegans were significantly decreased after exposure to ENIs(p<0.05);also the brood size and the lifespan of C. elegans were significantly reduced after exposure to ENIs(p<0.05). Moreover, after exposure to ENIs, the lifespan of mev-1 mutant hermaphrodites was shorter than that of wild type(p<0.05), indicating that mev-1 gene mutant could result in more sensitivity of the mutant hermaphrodites to ENIs exposure than the wild type. ENIs exposure also induced increase of germline cell apoptosis in time-dependent manner. With the prolonging of exposure time(>24h), the apoptotic germline cell increased significantly (compared with the control group, p<0.05).
Keywords:electrically generated negative air ions(ENIs)  Caenorhabditis elegans  development  lifespan  brood size
本文献已被 万方数据 等数据库收录!
点击此处可从《生态毒理学报》浏览原始摘要信息
点击此处可从《生态毒理学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号