| 检测细胞DNA断裂损伤效应的彗星实验法的改良 |
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| 引用本文： | 赖金龙,付倩,任莎,吴国,陶宗娅,张红,罗学刚.检测细胞DNA断裂损伤效应的彗星实验法的改良[J].生态毒理学报,2015,10(4):195-202. |
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| 作者姓名： | 赖金龙 付倩 任莎 吴国 陶宗娅 张红 罗学刚 |
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| 作者单位： | 1. 四川师范大学生命科学学院,成都,610101;2. 西南科技大学教育部生物质材料教育部工程研究中心,绵阳,621010 |
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| 基金项目： | 国家核设施退役及放射性废物治理科研重点项目(14ZG6101)；四川省教育厅重点课题(14ZA0030)；四川省生物质改性材料工程
技术研究中心(西南科技大学)开放课题(12zxbk03)；四川师范大学校级青年课题(14qn07)；四川师范大学实验技术课题(SYJS2014-14) |
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| 摘 要： | 为了解决彗星实验过程中常出现的脱胶、细胞核分离操作繁琐、重复性低等问题,对彗星实验方法进行了改良,初步建立了彗星实验的快速操作流程。结果显示,通过对载玻片进行预处理,可确保凝胶悬挂均匀;采用改良机械法分离的细胞核浓度适中;以0.5%(w/v)涂层琼脂糖作为基层、以1.5%(w/v)低熔点包埋琼脂糖作为叠加层的"双层凝胶法",辅以"推片法"铺胶,操作便捷且不发生脱胶现象;细胞核膜经裂解处理后再进行电泳和荧光观察,彗星图像清晰,杂质少。应用改良后的彗星实验方法,操作简便,耗时更短,实验效果良好,可快速检测出细胞DNA损伤效应。
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| 关 键 词： | 铯 蚕豆根尖细胞 遗传毒性 DNA损伤 彗星实验 改良 |
| 收稿时间： | 2014/11/25 0:00:00 |
| 修稿时间： | 2014/12/30 0:00:00 |
Improvements of the Comet Assay for Rapid Detecting the Rupture and Damage of DNA in Cells |
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| Lai Jinlong,Fu Qian,Ren Sh,Wu Guo,Tao Zongy,Zhang Hong and Luo Xuegang.Improvements of the Comet Assay for Rapid Detecting the Rupture and Damage of DNA in Cells[J].Asian Journal of Ecotoxicology,2015,10(4):195-202. |
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| Authors: | Lai Jinlong Fu Qian Ren Sh Wu Guo Tao Zongy Zhang Hong and Luo Xuegang |
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| Institution: | 1. Life Science College, Sichuan Normal University, Chengdu 610101, China
2. Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China;1. Life Science College, Sichuan Normal University, Chengdu 610101, China
2. Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China;1. Life Science College, Sichuan Normal University, Chengdu 610101, China
2. Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China;1. Life Science College, Sichuan Normal University, Chengdu 610101, China
2. Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China;1. Life Science College, Sichuan Normal University, Chengdu 610101, China
2. Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China;1. Life Science College, Sichuan Normal University, Chengdu 610101, China
2. Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China;1. Life Science College, Sichuan Normal University, Chengdu 610101, China
2. Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China |
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| Abstract: | |
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| Keywords: | Cs radicle cells Vicia faba genotoxicity DNA damage the comet assay improved |
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