二氧化锰颗粒对Hela细胞DNA损伤的尺度依赖性毒作用(英文)

为了观察二氧化锰颗粒物所致的尺度依赖性DNA损伤作用,将纳米尺度二氧化锰颗粒物(Nano-MnO2)和常规尺度二氧化锰颗粒物(Nor-MnO2)所致的DNA损伤进行了对比研究.将Hela细胞分别暴露于不同浓度(0、100、200、400μg·mL-1)的Nano-MnO2和Nor-MnO2中,染毒24h,采用彗星实验检测Hela细胞的DNA损伤水平.结果表明:与对照组相比,Nano-MnO2和Nor-MnO2均可使彗尾DNA百分比(TailDNA%)和尾矩(TailMoment)显著增加(p<0.01);而在同一浓度水平上,Nano-MnO2所致的DNA损伤则比Nor-MnO2所致的DNA损伤更为严重(p<0.01).结果提示:二氧化锰颗粒对Hela细胞DNA损伤具有尺度依赖性毒作用,纳米尺度比常规尺度二氧化锰颗粒毒作用更强烈.

Size-Dependent Toxicity of Dioxide Manganese Particles on DNA Damage in Hela Cells

A comparison was made for the size-dependent DNA damage induced by both nano dioxide manganese particles(Nano-MnO2)and normal size dioxide manganese particles(Nor-MnO2).Hela cells were exposed to different concentrations of both Nano-MnO2 and Nor-MnO2(0,100,200,400μg·mL-1),respectively.The DNA damage of Hela cells was measIIred by the comet assay after 24h Mno2-cultivated.Results showed that the Tail DNA%and Tail Momem were both significantly increased after exposed to both Nano-MnO2 and Nor-MnO2 when compared with the control(p<0.01 for all). At the same concenuation level,Nano-MnO2 could induce more serious DNA damage than Nor-Mn02(p<0.01 for all). The results suggested that Nano-MnO2 shollld have more serious toxic effect regarding to DNA damage of the Hela cells than that of Nor-Mno2.