邻苯二甲酸二乙基己酯(DEHP)对小白鼠肝脏毒性及脂质过氧化损伤

为研究邻苯二甲酸二乙基己酯(DEHP)对小鼠肝脏的毒性及脂质过氧化损伤作用机制,选择昆明4周龄小鼠80只,雌雄各半,随机分为4组。经食饵连续自然给食染毒,于染毒第4周末处死。测量小鼠肝脏和体重的变化,测定不同DEHP染毒剂量组小鼠的血液、肝脏中谷胱甘肽过氧化物酶(GPX)、过氧化氢(H2O2)和丙二醛(MDA),超氧化物歧化酶(SOD)的变化。将实验数据进行ANO-VA分析处理。结果表明,随着染毒剂量的增加,小鼠体重逐渐减少(p<0.01),高剂量组肝脏器系数明显上升(p<0.01)。苏木精-伊红染色法(简称HE染色)可见高剂量组肝脏组织有明显损伤。与对照组相比,DEHP3个剂量染毒组小鼠血液(75mg·kg-1组除外)及肝脏中GPX活性降低(p<0.05,p<0.01),H2O2含量增加(p<0.05,p<0.01);肝组织中(75mg·kg-1组除外)SOD活性降低(p<0.05,p<0.01),MDA含量增加(p<0.01)。以上结果说明DEHP对小鼠肝脏的毒性作用机制可能为脂质过氧化反应。

Hepatotoxic Effect and Lipid Oxidative Damage of Diethylhexyl Phthalate (DEHP) on Mice

To study the liver toxicity mechanism and lipid peroxidation induced by diethylhexyl phthalate (DEHP) on mice, eighty Kumming mice were divided into 4 groups randomly, which male was half. The animals were exposed to DEHP for 4 weeks. Then changes in liver and weight of the mice were detected. In the meantime, the activities of GPX and SOD, the contents of H2O2 and MDA in the blood and liver tissue were measured. The results of ANOVA test show that the weight of mice decreased significantly with increase of DEHP (p<0.01). The coefficient of liver/body with high dose group increased significantly. There were evident liver tissue damages in high dose group by HE staining. GPX activities of DEHP exposure groups in the blood tissue (except the 75 mg·kg-1 group) and liver tissue, the contents of H2O2, the contents of MDA and the activities of SOD in the liver tissue (except the 75 mg·kg-1 group) were significantly different(p<0.05,p<0.01) from the control group. Therefore, it was deduced that the liver toxicity mechanism of DEHP to mice was the lipid peroxidation.