| 气态甲醛致小鼠骨髓细胞DNA-蛋白质交联的研究 |
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| 引用本文： | 程文文,李兰,胡传禄,魏晨曦,杨旭,丁书茂.气态甲醛致小鼠骨髓细胞DNA-蛋白质交联的研究[J].生态毒理学报,2010,5(2):262-267. |
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| 作者姓名： | 程文文 李兰 胡传禄 魏晨曦 杨旭 丁书茂 |
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| 作者单位： | 华中师范大学,遗传调控与整合生物学湖北省重点实验室,武汉,430079;华中师范大学,遗传调控与整合生物学湖北省重点实验室,武汉,430079;华中师范大学,遗传调控与整合生物学湖北省重点实验室,武汉,430079;华中师范大学,遗传调控与整合生物学湖北省重点实验室,武汉,430079;华中师范大学,遗传调控与整合生物学湖北省重点实验室,武汉,430079;华中师范大学,遗传调控与整合生物学湖北省重点实验室,武汉,430079 |
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| 基金项目： | 国家科技支撑计划项目（No. 2006BAJ02A10） |
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| 摘 要： | 为探讨吸入性甲醛能否对小鼠骨髓造血细胞产生遗传毒性,以SPF级昆明雄性小鼠为材料,采用动态吸入方式连续染毒72h,取骨髓细胞,测定DNA-蛋白质交联.结果发现,随着甲醛浓度的升高(0、0.5、1.0、3.0mg·m-3),小鼠骨髓细胞DNA-蛋白质交联系数逐渐升高,0.5mg·m-3组与对照组存在显著差异(p<0.05),1.0、3.0mg·m-3组与对照组存在极显著差异(p<0.01).表明,在实验浓度范围内,甲醛对小鼠骨髓细胞具有一定的遗传毒性.
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| 关 键 词： | 甲醛 骨髓细胞 DNA-蛋白质交联 |
| 收稿时间： | 2009/4/30 0:00:00 |
| 修稿时间： | 2009/6/15 0:00:00 |
Gaseous Formaldehyde-Induced DNA-Protein Crosslinks in Mouse Bone Marrow Cells |
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| CHENG Wen-wen,LI Lan,HU Chuan-lu,WEI Chen-xi,YANG Xu and DING Shu-mao.Gaseous Formaldehyde-Induced DNA-Protein Crosslinks in Mouse Bone Marrow Cells[J].Asian Journal of Ecotoxicology,2010,5(2):262-267. |
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| Authors: | CHENG Wen-wen LI Lan HU Chuan-lu WEI Chen-xi YANG Xu and DING Shu-mao |
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| Institution: | Hubei Key laboratory of Genetic Regulation and Integrative Biology, Huazhong Normal University, Wuhan 430079 |
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| Abstract: | In order to study the genotoxicity of gaseous formaldehyde(FA)on mouse bone marrow cells,SPF level Kunming male mice were exposed to gaseous formaldehyde for 72h.KCl-SDS assay were applied to detect DNA-protein crosslinks(DPC)in bone marrow cells.Results showed that low concentration(0.5mg·m-3)gaseous formaldehyde could induce DPC formation significantly(compared with the control,p<0.05),and high concentrations(1.0,3.0mg·m-3)could induce DPC formation very significantly(p <0.01).DPC was increased with the increasing of gaseous formaldehyde concentrations(0.5,1.0,3.0mg·m-3).The results suggested that among the concentrations investigated in the present study,gaseous formaldehyde had obvious genetic toxicity on the mouse bone marrow cells. |
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| Keywords: | formaldehyde bone marrow DNA-protein crosslinks |
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