碲化镉量子点对小鼠睾丸组织的氧化损伤（Effects of CdTe Quantum Dots on the Antioxidant Enzymes Activity and Lipid Peroxidation in Testes of Mice）

为探讨碲化镉量子点(CdTeQDs)对小鼠睾丸的急性氧化损伤作用,将20只雄性ICR小鼠随机分成4组:对照组、1d、3d、7d(3个不同时间组),采用尾静脉注射进行一次性染毒2.5μmol·kg-1CdTeQDs,对照组注射等体积生理盐水.染毒后对睾丸的脏器系数以及组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性,丙二醛(MDA)含量分别进行测定,从而检测CdTeQDs对睾丸组织的急性氧化损伤作用.结果显示,与对照组相比,随染毒时间的延长,睾丸脏器系数无显著性变化(p>0.05);GSH-Px和SOD活性随染毒时间的延长呈逐渐升高趋势;而CAT活性呈逐渐降低趋势,且与对照组差异显著(p<0.01);MDA含量显著高于对照(p<0.01),且随时间变化不大.CdTeQDs染毒可导致小鼠睾丸组织氧化损伤,其损伤程度与染毒时间之间具有一定的时间-效应关系.

Effects of CdTe Quantum Dots on the Antioxidant Enzymes Activity and Lipid Peroxidation in Testes of Mice

The purpose of this study was to determine potential acute oxidative damage of cadmium telluride quantum dots(CdTe QD)to the mice testes.Twenty male mice were divided four groups:control and 1,3,7d exposure groups.The 1,3,7d exposure groups were intraperitoneally injected with CdTe QDs(2.5μmol·kg-1 body weight).Organ coefficients,the activity of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)and the content of malondialdehyde(MDA),as the indicator of lipid peroxidation,were measured.Results showed that compared with the control,no significant difference was found with the exposure time(p>0.05).GSH-Px and SOD activities increased gradually with exposure time.CAT activity decreased gradually with exposure time and significant difference was found between 7d exposure group and control(p<0.01).MDA contents were significantly higher than the control(p<0.01)and no significant difference was found with the exposure time(p>0.05).CdTe QD could induce oxidative damage to the mice testes and there was a time-dose relationship between the damage degree and exposure time.