双酚A及其类似物对斑马鱼胚胎及幼鱼的毒性效应

为探讨双酚A及其类似物对鱼类早期生长发育的毒性效应,研究了双酚A及其7种类似物对斑马鱼胚胎及仔鱼的毒性效应。通过对胚胎的孵化率、心率、仔鱼体长等指标进行测定分析,结果显示:(1)双酚A及其7种类似物都可使斑马鱼胚胎出现心包水肿、卵黄囊肿、脊柱弯曲和尾部弯曲等症状。(2)综合考虑斑马鱼78 hpf胚胎心率、120 hpf胚胎孵化率及7 dpf仔鱼体长抑制率等指标,8种受试物中双酚P(BPP)的毒性最大,其次是双酚AP(BPAP)、双酚AF(BPAF)、双酚Z(BPZ),然后是双酚A(BPA)、双酚B(BPB)、双酚F(BPF),双酚S(BPS)的毒性最小,每种受试物的浓度与受精卵的孵化率、仔鱼心率呈明显负相关关系,与体长抑制率呈明显正相关关系。8种受试物毒性与辛醇-水分配系数(log K_(OW))呈正相关关系,log K_(OW)越大毒性则越大。

Effects of Bisphenol A and Its Analogues on Zebrafish Embryos and Larvae

We evaluated the toxic effect of bisphenol A (BPA) and its seven analogues on zebrafish embryos and larvae. Based on the results of the hatching rate (120 hpf), heart rate (78 hpf), body length of larvae (7 dpf), it is indicated that: (1) BPA and its analogues caused cardiac edema, cyst vitelline, spinal curvature and tail bending; (2) the order of the toxicity is BPP> BPAP> BPAF> BPZ> BPA> BPB> BPF> BPS. The concentrations of the test substances were negatively correlated with the embryo hatching rate and the heart rate of larvae, while positive correlation with inhibition rate of larval length. In addition, the toxicity of these eight kinds of BPA analogues and the values of octanol-water partition (log KOW) were positively correlated.