芴对斑马鱼不同发育阶段毒性效应的比较

为考察多环芳烃芴对斑马鱼的毒性效应,将斑马鱼成鱼以及0 hpf(孵化后0 h)和10 hpf(孵化后10 h)的斑马鱼胚胎分别暴露于不同浓度的芴溶液中,观察各时间段半数致死浓度(LC50)及各毒理学终点的半数效应浓度(EC50)。结果表明,成鱼、0hpf胚胎、10 hpf胚胎的48 h-LC50分别为4.013、6.074、28.980 mg·L-1,三者对芴的致死敏感性为成鱼0 hpf胚胎10 hpf胚胎。芴的毒性作用主要发生在胚胎的分节期(10 h)之前,0 hpf胚胎各项指标敏感性依次为:36 h心率异常48 h心包囊肿24 h无主动运动48 h卵黄囊水肿24 h发育阻滞。斑马鱼对芴染毒最敏感指标为36 h心率异常,0 hpf胚胎心率值随染毒浓度增大而减小,并在8 mg·L-1达到抑制峰值,心率最大抑制率为41.3%。芴主要作用于斑马鱼胚胎心脏器官组织。

Comparison of Toxic Effect of Fluorene on Zebrafish at Different Developmental Stages

In order to study the toxic effect of fluorene on zebrafish, adult zebrafish, 0 hpf (hours past fertilization) embryo and 10 hpf embryo were exposed to different concentrations of fluorene aqueous solution. The values of median lethal concentration (LC₅₀) at different time intervals and median effective concentration (EC₅₀) for different toxicological endpoints were calculated. The results showed that the 48 h-LC₅₀ of adult zebrafish, 0 hpf embryo and 10 hpf embryo were 4.013, 6.074 and 28.980 mg·L^-1, respectively, which indicated that the sensitivity of zebrafish to fluorene was in the order of adult>0 hpf embryo>10 hpf embryo. The impact of fluorene mainly occurred before embryo segmentation (10 h). The sensitivity of each toxicity endpoint for 0 hpf embryo was in the order of 36 h heart rate abnormality>48 h pericardial cyst>24 h inactive sports>48 h yolk cyst>24 h development block. The 36 h heart rate abnormality was the most sensitive indicator, and the 36 h heart rate of 0 hpf embryo decreased with increasing fluorine concentration. The peak suppression ratio of heart rate was 41.3%, which occurred at 8 mg·L^-1. Cardiac tissues were the main target organs of fluorene.