镧、铈、钕对小鼠肝细胞核的氧化损伤作用

轻稀土元素进入生物体后主要累积于肝脏,进入肝细胞,分布于细胞核上.为探讨轻稀土元素对小鼠肝细胞核的氧化损伤作用,选用5周龄雄性封闭群(ICR)小鼠灌喂10、20和40 mg·kg-1的稀土元素镧(La)、铈(Ce)和钕(Nd),6周后测定小鼠肝细胞核中超氧化物岐化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(...

Oxidative Damage Induced by Lanthanum, Cerium and Neodymium in Liver Nuclei of Mice

Light rare earth elements are found to mainly accumulate in the livers of receiving organisms. They can enter into hepatocytes and are mainly distributed in the nuclei. To investigate the oxidative damage effects of light rare earth elements on the hepatocyte nuclei isolated from the mouse liver, five-week-old male ICR (imprinting control region) mice were exposed to lanthanum (La), cerium (Ce) and neodymium (Nd) by oral gavage for 6 weeks with different doses, i.e., 10, 20 and 40 mg·kg^-1. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were determined as well as the contents of glutathione (GSH) and malondialdehyde (MDA). ANOVA analysis of the results showed that compared with the control group, GPx activity and MDA content in every exposure group increased (p<0.05); except in the Ce low-dosage group, SOD and CAT activities in other exposure groups decreased (p<0.05) in a dose-dependent manner; except in the La low-dosage, Ce low-dosage and Ce middle-dosage groups, GSH content in other exposure groups increased (p<0.05). It is indicated that La, Ce and Nd could induce oxidative damage in hepatic nuclei of mice.