O3浓度升高对不同生长期冬小麦叶片抗氧化系统的影响

由于人口的快速增长,人类活动导致近地层O3浓度不断提高,O3浓度升高将对植物、动物和人体健康产生极大的危害.采用开顶式气室(OTC)原位实验方法,研究O3浓度升高对不同生长期冬小麦叶片抗氧化系统的影响,进而分析O3对植物的伤害机制.结果表明,O3浓度升高可导致冬小麦拔节期和抽穗期叶片超氧化物歧化酶(SOD)、过氧化物酶...

Effects of Elevated Ozone on Antioxidant System of Winter Wheat Leaf in Varied Growth Stages

As human population increases rapidly, human activities cause the increase of tropospheric ozone (O₃) which significantly exerts adverse effects on the health of plants, animals and human beings. In this paper, open top chambers (OTCs) under field conditions were used to investigate the antioxidant system (malondialdehyde, MDA; soluble protein; ascorbic acid, AsA; glutathione, GSH, superoxide dismutase, SOD; peroxidase, POD; catalase, CAT; ascorbate peroxidase, APX; glutathione reductase, GR) of winter wheat leaf in different growing stages under elevated O₃. The results indicated that the increase of O₃ significantly affected the antioxidant system of winter wheat leaf both in jointing stage and heading stage. In the level of 120 nL·L^-1, as compared with the control group, the activities of SOD, CAT and APX of winter wheat leaf in jointing stage decreased by 50.3%, 64.9% and 75.5%, while the POD and GR activity increased by 45.1% and 80.5%, respectively. The contents of MDA, AsA and GSH increased by 314.3%, 8.4% and 31.7%, respectively, and the content of soluble protein decreased by 47.5%. Different levels of O₃ and different growing stages of winter wheat both resulted in different changes of substances in the antioxidant system. Under the stress of O₃ pollution, the antioxidant system of winter wheat had an extremely complex change in order to prevent the damage caused by active oxygen species (AOS), which is the self-regulation of plants.