体育场馆内PM_(2.5)暴露对运动大鼠行为学及相关无氧代谢酶活性的影响

通过非暴露式气管滴注法建立损伤模型,按照低、中、高3种剂量细颗粒物(PM_(2.5))进行染毒,以研究不同浓度PM_(2.5)对运动大鼠行为学及部分无氧代谢酶活性的影响。实验选取32只雄性Wistar SPF(specific pathogen free,SPF)大鼠随机分为运动对照组(EC)、高剂量运动组(30 mg·kg~(-1))(HPE)、中剂量运动组(15 mg·kg~(-1))(MPE)、低剂量运动组(7.5 mg·kg~(-1))(LPE),利用卒中指数和神经病学症状评分对大鼠的行为学进行评价,通过酶联免疫法(ELISA)测定大鼠血清、肺泡灌洗液(BALF)以及股四头肌组织中己糖激酶(HK)、丙酮酸激酶(PK)、磷酸果糖激酶(PFK-1)的活性。结果表明,与EC相比,3个剂量暴露组中,卒中指数和神经病学症状评分差异均有统计学意义;LPE、MPE、HPE组各组织中HK、PK、PFK-1活性均下降,差异有统计学意义(P0.05或P0.01)。综上所述,急性PM_(2.5)暴露可对大鼠行为学产生不利的影响,使大鼠部分组织的无氧代谢酶酶活性降低,影响机体的运动能力。

The Effects of PM2.5 Exposure on the Behavior of Sports Rats and the Activity of Anaerobic Metabolic Enzymes in the Stadium

Discuss the mechanism of PM2.5 to sugar metabolism through studying the influence of different con-centration of PM2.5 on incremental exercise rats. The 32 male Wistar SPF (specific pathogen free, SPF) rats were randomly selected and divided into control group (EC), high dose of PM2.5+exercise group (HPE), middle dose of PM2.5+exercise group (MPE) and low dose of PM2.5+ exercise group (LPE), and PM2.5 was administered to rats by intratracheal instillation at the doses of 30 mg·kg-1 bw, 15 mg·kg-1 bw and 7.5 mg·kg-1 bw respectively. Then all rats were trained by treadmill running with increasing load. Stroke index score and neurologic symptoms were used to evaluate the rats' behavior, and the HK, PK, PFK-1 were tested by ELISA. The stroke index were statistically significant (P<0.05 or P<0.01) in three group, compared with the control group;as a result, the activity of HK, PK, PFK-1 were declined in LPE, MPE, HPE compared with the EC, and the difference was statisti-cally significant. The PM2.5 exposure can affect rats' behavior, resulting in changes of the anaerobic metabolizing enzymes, and then reducing the body ' s exercise capacity. With the increase of PM2.5 concentration, HK, PK, PFK-1 activity decreased.